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Our Abpromise guarantee covers the use of ab38689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/4000. Predicted molecular weight: 27 kDa.|
|IHC-P||1/50 - 1/100.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling GFP with ab38689. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-mouse polyclonal (ready to use) was used as the secondary antibody. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter.
Immunohistochemistry (Frozen sections) analysis of E10.5 mouse embryo tissue sections labeling GFP with ab38689 at 1/200 dilution. The tissue was fixed with paraformaldehyde and permeabilized with PBS / 0.5% v/v Triton X-100. The tissue was incubated with ab38689 in 1% BSA + 5% goat serum + PBST for 12 hours at 25°C. A polyclonal goat anti-mouse Alexa Fluor® 568 secondary antibody was used at 1/1000 dilution.
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
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