概述

  • 产品名称Anti-GFI1抗体
    参阅全部 GFI1 一抗
  • 描述
    兔多克隆抗体to GFI1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human GFI1.

    (Peptide available as ab22461.)

  • 阳性对照
    • THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab21061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能Transcription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription.
  • 疾病相关Defects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
    Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
  • 序列相似性Contains 6 C2H2-type zinc fingers.
  • 结构域The Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes.
  • 翻译后修饰Ubiquitinated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GFI 1 antibody
    • GFI1 antibody
    • GFI1_HUMAN antibody
    • Growth factor independence 1 antibody
    • Growth factor independent 1 antibody
    • Growth factor independent protein 1 antibody
    • SCN2 antibody
    • Zinc finger protein 163 antibody
    • Zinc finger protein Gfi 1 antibody
    • Zinc finger protein Gfi-1 antibody
    • Zinc finger protein Gfi1 antibody
    • ZNF 163 antibody
    • ZNF163 antibody
    see all

Anti-GFI1 antibody 图像

  • All lanes : Anti-GFI1 antibody (ab21061) at 1 µg/ml

    Lane 1 : THP1 whole cell lysate (ab7913)
    Lane 2 : THP1 whole cell lysate (ab7913) with Human GFI1 peptide (ab22461) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680)

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa (possible cleavage fragment,cross reactivity).

    See Abreview

Anti-GFI1 antibody (ab21061)参考文献

This product has been referenced in:
  • Moignard V  et al. Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis. Nat Cell Biol 15:363-72 (2013). Read more (PubMed: 23524953) »
  • Khandanpour C  et al. The human GFI136N variant induces epigenetic changes at the Hoxa9 locus and accelerates K-RAS driven myeloproliferative disorder in mice. Blood 120:4006-17 (2012). Mouse . Read more (PubMed: 22932805) »

See all 4 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (Hematopoietic progenitors)
Specification Hematopoietic progenitors
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Paraformaldehyde
Detection step Real-time PCR
Positive control GFI-1 locus
Negative control Alpha actin locus
Username

Abcam user community

Verified customer

提交于 Jun 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL-60, Jurkat, Raw264.7, PC12)
Loading amount 20 µg
Specification HL-60, Jurkat, Raw264.7, PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"