Anti-GFAP抗体[G-A-5] (Cy3 ®) (ab49874)

概述

  • 产品名称
    Anti-GFAP抗体[G-A-5] (Cy3 ®)
    参阅全部 GFAP 一抗
  • 描述
    小鼠单克隆抗体[G-A-5] to GFAP (Cy3 ®)
  • 偶联物
    Cy3 ®. Ex: 552nm, Em: 565nm
  • 特异性
    Recognizes an epitope localized on the carboxy terminal Cys II fragment of GFAP and the N-terminal part of the tail sequence of the molecule.
  • 经测试应用
    适用于: IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Cat, Human, Pig, Goldfish
  • 免疫原

    Purified GFAP from pig spinal cord

  • 阳性对照
    • Rat cerebellum.
  • 常规说明

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) conjugated to Cy3®. The Cy3®-antibody conjugate is extensively dialyzed to remove unbound Cy3®.

性能

应用

Our Abpromise guarantee covers the use of ab49874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/400.
IHC-P 1/400. The epitope recognized by the antibody is partially sensitive to prolonged formalin fixation, but is resistant to alcohol-fixation followed by paraffin-embedding and to acetone-fixation of frozen sections.
ICC/IF Use at an assay dependent dilution. PubMed: 20211723

靶标

  • 功能
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性
    Expressed in cells lacking fibronectin.
  • 疾病相关
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylated by PKN1.
  • 细胞定位
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

图片

  • Immunocytochemistry/Immunofluorescence analysis of chicken astrocytes labelling GFAP (red) with ab49874. Nuclei stained using DAPI (blue). Cells were fixed with methanol for 15 minutes, washed in PBS and permeabilized with PBST for 10 minutes. Cells were then washed and blocked using 1% BSA in PBST for 30 minutes. Cells were incubated with the primary antibody (1:400 in blocking solution) for 1 hour at room temperature or overnight in a humid chamber in 4°C. 

文献

This product has been referenced in:
  • Liu Y  et al. PD-L1 expression by neurons nearby tumors indicates better prognosis in glioblastoma patients. J Neurosci 33:14231-45 (2013). Read more (PubMed: 23986257) »
  • Dotan S  et al. An avian model for the reversal of neurobehavioral teratogenicity with neural stem cells. Neurotoxicol Teratol 32:481-8 (2010). ICC/IF ; Chicken . Read more (PubMed: 20211723) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Acetone
Permeabilization
No
Blocking step
PBS +1% BSA +1% Ovalbumine +1% goat serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
Username

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Verified customer

提交于 Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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