Anti-GFAP抗体- Astrocyte Marker, prediluted (ab929)

概述

  • 产品名称Anti-GFAP抗体- Astrocyte Marker, prediluted
    参阅全部 GFAP 一抗
  • 描述
    兔多克隆抗体to GFAP - Astrocyte Marker, prediluted
  • 特异性In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
  • 经测试应用适用于: ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Cat, Human, Kangaroo, Monkey
  • 免疫原

    Glial fibrillary acidic protein isolated from cow spinal cord.

  • 阳性对照
    • ICC/IF: U-87 MG cells.
  • 常规说明GFAP is Glial Fibrillary Acidic Protein.

性能

应用

Our Abpromise guarantee covers the use of ab929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 18365011
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性Expressed in cells lacking fibronectin.
  • 疾病相关Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰Phosphorylated by PKN1.
  • 细胞定位Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody - Astrocyte Marker, prediluted 图像

  • ab929 staining GFAP in neural progenitor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% formaldehydum polymerisatum. Samples were incubated with primary antibody diluted 1/6 overnight at 4°C, and then incubated with the secondary FITC-conjugated antibodies for 1 h at room temperature.

  • ab929 stained U-87 MG cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab929 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Ab929 staining human substantia nigra. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1-4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-GFAP antibody - Astrocyte Marker, prediluted (ab929)参考文献

This product has been referenced in:
  • Wang B  et al. Nogo-66 promotes the differentiation of neural progenitors into astroglial lineage cells through mTOR-STAT3 pathway. PLoS ONE 3:e1856 (2008). ICC/IF ; Rat . Read more (PubMed: 18365011) »
  • Zhang J  et al. Olfactory ensheathing cells promote proliferation and inhibit neuronal differentiation of neural progenitor cells through activation of Notch signaling. Neuroscience 153:406-13 (2008). ICC/IF ; Rat . Read more (PubMed: 18400409) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

After completing a short literature search, GFAP is commonly used as an protoplasmic astrocyte marker. Below are two references:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00382...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Chicken Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - trit 100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Aug 25 2010

Thank you for your enquriy. It is very difficult to pinpoint exact IgG conc. for prediluted antibodies. Though, the condentration of ab929 is in the range of 1-4 ug/ml. I hope this information helps you, and please do not hesitate to contact us i...

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Thank you for your enquiry. We are sorry to hear that the customer is having problem with this antibody. It depends on the thickness of the section. 4-5 um think sections in 100% ice cold acetone for 10-15 minutes. If you need anything further or any ...

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Thank you for your enquiry. We are very sorry to hear that you are having problem with this antibody. We have sold several vials of this antibody and do not have any complaint. We would suggest running the staining again; it is always a good idea...

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The concentration is approx. 1000 ug/ml. Molecular Weight is 52 kDa.

We have the following GFAP antibodies, undiluted, which may suit your needs: ab7260 - Rabbit polyclonal to GFAP - Astrocyte Marker ab7806 - Mouse monoclonal [GF-01] to GFAP - Astrocyte Marker ab8136 - Mouse monoclonal [2A5] to GFAP - Astrocyte...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"