概述

  • 产品名称
  • 描述
    羊多克隆抗体to GFAP
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    H-CKLADVYQAELRELRLRLDQ-OH

    conjugated to KLH, corresponding to amino acids in GFAP. This sequence is conseverd in Human Mouse and Rat.

  • 阳性对照
    • IHC-P and ICC/IF: Human and Rat brain tissue WB: Human brain homogenate

性能

应用

Our Abpromise guarantee covers the use of ab90601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Predicted molecular weight: 50 kDa.
ELISA 1/100 - 1/10000.
IHC-P 1/4000 - 1/8000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性
    Expressed in cells lacking fibronectin.
  • 疾病相关
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylated by PKN1.
  • 细胞定位
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

图片

  • ab90601, at a 1/4000 dilution, staining GFAP in paraffin embedded Human brain tissue (left image) by Immunohistochemistry. Right image shows negative control: 40X objective.
  • ab90601, at a 1/6000 dilution, staining GFAP in Rat brain tissue (left image) by Immunohistochemistry. Right image shows negative control, 40X objective.
  • ab90601, at a 1/500 dilution, staining GFAP in Human brain tissue by Immunofluorescence. 40X objective.
  • ab90601, at a 1/500 dilution, staining GFAP in Rat brain by Immunofluorescence. 40X objective.
  • Anti-GFAP antibody (ab90601) at 1/6000 dilution + Human brain homogenate at 5 µg

    Predicted band size : 50 kDa

文献

This product has been referenced in:
  • Wüthrich C & Koralnik IJ Frequent infection of cortical neurons by JC virus in patients with progressive multifocal leukoencephalopathy. J Neuropathol Exp Neurol 71:54-65 (2012). IHC-P ; Human . Read more (PubMed: 22157619) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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