Anti-GFAP抗体(ab90601)
Key features and details
- Sheep polyclonal to GFAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GFAP抗体
参阅全部 GFAP 一抗 -
描述
羊多克隆抗体to GFAP -
宿主
Sheep -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Cow, Cynomolgus monkey -
免疫原
Synthetic peptide:
H-CKLADVYQAELRELRLRLDQ-OH
conjugated to KLH, corresponding to amino acids in GFAP. This sequence is conseverd in Human Mouse and Rat. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 50ul water and then 50ul glycerol, gently vortexing. Once resuspended, the serum will be yellow in colour. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab90601于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/10000. Predicted molecular weight: 50 kDa.
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IHC-P |
1/4000 - 1/8000.
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ICC/IF |
1/500 - 1/1000.
|
说明 |
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WB
1/5000 - 1/10000. Predicted molecular weight: 50 kDa. |
IHC-P
1/4000 - 1/8000. |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
组织特异性
Expressed in cells lacking fibronectin. -
疾病相关
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylated by PKN1. -
细胞定位
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 281189 Cow
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: Q28115 Cow
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
see all -
别名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
图片
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ab90601, at a 1/4000 dilution, staining GFAP in paraffin embedded Human brain tissue (left image) by Immunohistochemistry. Right image shows negative control: 40X objective.
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ab90601, at a 1/6000 dilution, staining GFAP in Rat brain tissue (left image) by Immunohistochemistry. Right image shows negative control, 40X objective.
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ab90601, at a 1/500 dilution, staining GFAP in Human brain tissue by Immunofluorescence. 40X objective.
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ab90601, at a 1/500 dilution, staining GFAP in Rat brain by Immunofluorescence. 40X objective.
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Anti-GFAP antibody (ab90601) at 1/6000 dilution + Human brain homogenate at 5 µg
Predicted band size: 50 kDa
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab90601 被引用在 5 文献中.
- Griffiths B et al. Inhibition of microRNA-200c preserves astrocyte sirtuin-1 and mitofusin-2, and protects against hippocampal neurodegeneration following global cerebral ischemia in mice. Front Mol Neurosci 15:1014751 (2022). PubMed: 36466801
- Liu H et al. Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions. Nat Commun 12:114 (2021). PubMed: 33414464
- Wüthrich C et al. JC Virus Infects Neurons and Glial Cells in the Hippocampus. J Neuropathol Exp Neurol 75:712-717 (2016). PubMed: 27297673
- Wüthrich C et al. Lack of Major Histocompatibility Complex Class I Upregulation and Restrictive Infection by JC Virus Hamper Detection of Neurons by T Lymphocytes in the Central Nervous System. J Neuropathol Exp Neurol 74:791-803 (2015). PubMed: 26115192
- Wüthrich C & Koralnik IJ Frequent infection of cortical neurons by JC virus in patients with progressive multifocal leukoencephalopathy. J Neuropathol Exp Neurol 71:54-65 (2012). IHC-P ; Human . PubMed: 22157619