Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.
1/1000 - 1/5000.
Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.
功能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
组织特异性Expressed in cells lacking fibronectin.
疾病相关Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
序列相似性Belongs to the intermediate filament family.
翻译后修饰Phosphorylated by PKN1.
细胞定位Cytoplasm. Associated with intermediate filaments.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GFAP antibody (ab4674)This image is a courtesy of Ben Deverman
ab4674 staining GFAP in mouse hippocampus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 4% PFA and blocking with 10% serum for 30 minutes at 250C was performed. The sample was incubated with primary antibody (1/500) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to chicken IgG was used as secondary antibody at 1/400 dilution. Staining was intensified with 2-3 minutes of retrieval with trypsin at room temperature.
Rat astrocytes stained with ab4674 (red). The anti-GFAP antibody was used at a dilution of 1:50 from affinity purified material using our standard fixation and staining procedure (in protocol section). Hoechst dye reveals nuclear DNA in blue.
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab4674)This image is courtesy of an anonymous Abreview
ab4674 staining GFAP in rat primary astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% serum for 20 minutes at 20°C. Samples were incubated with primary antibody (1/2000) for 24 hours at 4°C. ab6873, a goat anti-chicken IgY FITC (1/1000) was used as the secondary antibody.
All lanes : Anti-GFAP antibody (ab4674) at 1/5000 dilution
Lane 1 : Whole rat brain extract Lane 2 : Mouse brain extract
Predicted band size : 50 kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GFAP antibody (ab4674)This image is courtesy of an Abreview submitted by Alex Zagariya
ab4674 staining GFAP in Rabbit eye tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with BSA for 2 hours at 4°C. The sample was incubated with primary antibody (1/1000) at 4°C for 12 hours. ab150175, a goat anti-chicken IgY Alexa Fluor® 647 (1/1000), was used as the secondary antibody.
Flow Cytometry - Anti-GFAP antibody (ab4674)This image is courtesy of an anonymous Abreview
ab4674 staining GFAP - Astrocyte Marker in Human Brain cells by Flow Cytometry. Cells were grown in stem cell media, RHB-A, and collected using 0.05% trypsin + trypsin inhibitor. Cells were fixed in 80% Acetone for 5-10 minutes at -20°C. The sample was incubated with the primary antibody (1/100 in 10% FCS in PBS) for 20 minutes at 25°C. ab46969 a goat anti-chicken IgY FITC (1/100) was used as the secondary antibody Gating Strategy: Cells expressing GFAP
ELISA - Anti-GFAP antibody (ab4674)This image is courtesy of an anonymous Abreview
ab4674 detecting recombinant GFAP-Astrocyte Marker by direct ELISA. Mouose recombinat GFAP Protein was coated onto a microplate in carbonate coating buffer pH 9.6 for 1 hour at 37°C. Plate were blocked with 3% BSA for 1 hour at 37°C and incubated with the primary antibody (1/5000 in PBS + 1% Tween-20) for 1 hour at 37°C. An undiluted alkaline phosphatase Goat anti-chicken IgG polyclonal was used as the secondary antibody.