概述

  • 产品名称
  • 描述
    鸡多克隆抗体to GFAP
  • 经测试应用
    适用于: ELISA, IHC-Fr, IHC-FoFr, IHC-P, Flow Cyt, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Human, Rhesus monkey, Apteronotus leptorhynchus
    预测可用于: Mammal
  • 免疫原

    Full length native protein (purified) corresponding to Cow GFAP.

  • 阳性对照
    • IHC-P: FFPE mouse brain normal. IHC-P: FFPE rat hippocampus normal.

性能

应用

Our Abpromise guarantee covers the use of ab4674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-Fr 1/1000 - 1/5000. Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.
IHC-FoFr 1/1000 - 1/5000. PubMed: 20098733Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab37382 - Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.

Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.

ICC/IF 1/1000 - 1/5000.

Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.

PubMed: 25418722

靶标

  • 功能
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性
    Expressed in cells lacking fibronectin.
  • 疾病相关
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylated by PKN1.
  • 细胞定位
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody 图像

  • IHC image of GFAP staining in a formalin fixed, paraffin embedded normal rat hippocampus tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was incubated with ab4674 at 1/1000 dilution for 15 mins at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

  • IHC image of GFAP staining in a formalin fixed, paraffin embedded mouse normal brain tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was incubated with ab4674 at 1/1000 dilution for 15 mins at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

  • Anti-GFAP antibody (ab4674) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg

    Secondary
    AlexaFluor®488-conjugated goat anti-chicken polyclonal IgG at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

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  • ab4674 staining GFAP in mouse hippocampus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 4% PFA and blocking with 10% serum for 30 minutes at 250C was performed. The sample was incubated with primary antibody (1/500) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to chicken IgG was used as secondary antibody at 1/400 dilution. Staining was intensified with 2-3 minutes of retrieval with trypsin at room temperature.

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  • Rat astrocytes stained with ab4674 (red). The anti-GFAP antibody was used at a dilution of 1:50 from affinity purified material using our standard fixation and staining procedure (in protocol section). Hoechst dye reveals nuclear DNA in blue.
  • ab4674 staining GFAP in rat primary astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% serum for 20 minutes at 20°C. Samples were incubated with primary antibody (1/2000) for 24 hours at 4°C. ab6873, a goat anti-chicken IgY FITC (1/1000) was used as the secondary antibody.

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  • All lanes : Anti-GFAP antibody (ab4674) at 1/5000 dilution

    Lane 1 : Whole rat brain extract
    Lane 2 : Mouse brain extract


    Predicted band size : 50 kDa
  • ab4674 staining GFAP in Rabbit eye tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with BSA for 2 hours at 4°C. The sample was incubated with primary antibody (1/1000) at 4°C for 12 hours. ab150175, a goat anti-chicken IgY Alexa Fluor® 647 (1/1000), was used as the secondary antibody.

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  • ab4674 staining GFAP - Astrocyte Marker in Human Brain cells by Flow Cytometry. Cells were grown in stem cell media, RHB-A, and collected using 0.05% trypsin + trypsin inhibitor. Cells were fixed in 80% Acetone for 5-10 minutes at -20°C. The sample was incubated with the primary antibody (1/100 in 10% FCS in PBS) for 20 minutes at 25°C. ab46969 a goat anti-chicken IgY FITC (1/100) was used as the secondary antibody
    Gating Strategy: Cells expressing GFAP

    See Abreview

  • ab4674 detecting recombinant GFAP-Astrocyte Marker by direct ELISA. Mouose recombinat GFAP Protein was coated onto a microplate in carbonate coating buffer pH 9.6 for 1 hour at 37°C. Plate were blocked with 3% BSA for 1 hour at 37°C and incubated with the primary antibody (1/5000 in PBS + 1% Tween-20) for 1 hour at 37°C. An undiluted alkaline phosphatase Goat anti-chicken IgG polyclonal was used as the secondary antibody.

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Anti-GFAP antibody (ab4674)参考文献

This product has been referenced in:
  • Benford H  et al. A sweet taste receptor-dependent mechanism of glucosensing in hypothalamic tanycytes. Glia 65:773-789 (2017). ICC/IF ; Rat . Read more (PubMed: 28205335) »
  • Moeendarbary E  et al. The soft mechanical signature of glial scars in the central nervous system. Nat Commun 8:14787 (2017). ICC/IF ; Rat . Read more (PubMed: 28317912) »

See all 106 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 4°C
Antigen retrieval step
None
Sample
Rabbit Tissue sections (eye)
Specification
eye
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Alex Zagariya

Verified customer

提交于 Dec 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate Gel)
Loading amount
15 µg
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Mar 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Cerebrum & Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Cerebrum & Cerebellum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Kathleen

Verified customer

提交于 Dec 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory stem cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory stem cells GFP)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells GFP
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 25 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells (neuronal indicution))
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells (neuronal indicution)
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

提交于 Sep 20 2016

Application
IHC - Wholemount
Sample
Mouse Tissue (Colon)
Specification
Colon
Username

Abcam user community

Verified customer

提交于 Jun 13 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - 50% ethanol, 15min
Specification
Spinal cord
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 11 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Mouse hippocampus)
Specification
Mouse hippocampus
Permeabilization
Yes - 0.2% Triton X100
Fixative
Paraformaldehyde
Username

gergely kovacs

Verified customer

提交于 Mar 18 2015

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