Anti-GFAP抗体(ab16997)
Key features and details
- Rabbit polyclonal to GFAP
- Suitable for: ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-GFAP抗体
参阅全部 GFAP 一抗 -
描述
兔多克隆抗体to GFAP -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Full length native protein (purified) corresponding to Cow GFAP. Isolated from cow spinal cord.
-
常规说明
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
-
纯度
IgG fraction -
Primary antibody说明
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16997于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
1/100.
|
|
IHC-P | (1) |
1/50 - 1/250.
|
说明 |
---|
ICC
1/100. |
IHC-P
1/50 - 1/250. |
靶标
-
功能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
组织特异性
Expressed in cells lacking fibronectin. -
疾病相关
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylated by PKN1. -
细胞定位
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
-
数据库链接
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- Unigene: 514227 Human
- Unigene: 1239 Mouse
-
别名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
图片
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue, staining GFAP with ab16997.
-
Mouse cortical primary astrocytes stained for GFAP using ab16997 at a dilution of 1/100 in ICC/IF.
-
Formalin-fixed, paraffin-embedded section of human brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.
实验方案
数据表及文件
-
Datasheet download
文献 (57)
ab16997 被引用在 57 文献中.
- Pietiläinen O et al. Astrocytic cell adhesion genes linked to schizophrenia correlate with synaptic programs in neurons. Cell Rep 42:111988 (2023). PubMed: 36640364
- Yan J et al. Met-RANTES preserves the blood-brain barrier through inhibiting CCR1/SRC/Rac1 pathway after intracerebral hemorrhage in mice. Fluids Barriers CNS 19:7 (2022). PubMed: 35062973
- Curreli S et al. Complementary encoding of spatial information in hippocampal astrocytes. PLoS Biol 20:e3001530 (2022). PubMed: 35239646
- Molnár K et al. Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury. J Neuroinflammation 19:68 (2022). PubMed: 35305649
- Mehlhorn J et al. Regional Patterning of Adult Neurogenesis in the Homing Pigeon's Brain. Front Psychol 13:889001 (2022). PubMed: 35898980