概述

  • 产品名称
  • 描述
    兔多克隆抗体to GFAP
  • 特异性
    ab116010 distinguishes neoplasm of astrocytes origin from other neoplasm of the CNS.
  • 经测试应用
    适用于: IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Goat, Guinea pig, Cow, Human, Pig
  • 免疫原

    Purified Human GFAP protein.

  • 阳性对照
    • Human brain or astrocytoma tissues. This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH

性能

应用

Our Abpromise guarantee covers the use of ab116010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
ICC 1/50 - 1/200.
ICC/IF 1/1000.

靶标

  • 功能
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性
    Expressed in cells lacking fibronectin.
  • 疾病相关
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylated by PKN1.
  • 细胞定位
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

图片

  • ICC/IF image of ab116010 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab116010 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

ab116010 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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