Anti-GFAP抗体[6F2] (ab8975)

概述

  • 产品名称Anti-GFAP抗体[6F2]
    参阅全部 GFAP 一抗
  • 描述
    小鼠单克隆抗体[6F2] to GFAP
  • 特异性Reacts exclusively with glial fibrillary acidic protein which is present in astrocytes in the central nervous system and Schwann cells.
  • 经测试应用适用于: IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Glial fibrillary acidic protein (full length) from human brain.

性能

应用

Our Abpromise guarantee covers the use of ab8975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/100 - 1/1000.

靶标

  • 功能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性Expressed in cells lacking fibronectin.
  • 疾病相关Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰Phosphorylated by PKN1.
  • 细胞定位Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody [6F2] 图像

  • GFAP immunogold labelling TEM of corticobasal degeneration brain tissue showing heavy and highly-specific labelling over a glial process. Bar =
    100 nm.

    This picture was kindly supplied as part of the review submitted by Dr Julian Thorpe (University of Sussex).

Anti-GFAP antibody [6F2] (ab8975)参考文献

ab8975 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. For ab8975, the antibody was prepared by immunizing with a GFAP preparation containing the full length protein. You also enquired about ab7806 - the epitope has not been mapped. Please contact us again if you have any add...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (cultured neurones)
Specification cultured neurones
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 Dec 20 2005

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cell (rat astrocyte culture)
Specification rat astrocyte culture
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 Dec 20 2005

We can recommend to try a dilution of 1/100 but please remember to try a range of dilutions in a preliminary experiment to determine what dilution is best suited for your samples. You may also find the following reference useful: 1. Van Muijen, G. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"