概述

  • 产品名称Anti-GFAP抗体[2A5]
    参阅全部 GFAP 一抗
  • 描述
    小鼠单克隆抗体[2A5] to GFAP
  • 经测试应用适用于: Sandwich ELISA, ICC, IHC-P, ICC/IF, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Pig
    预测可用于: Mammal不与反应: Chicken
  • 免疫原

    Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.

  • 阳性对照
    • Homogenized cow, pig, human, rat, mouse or other mammalian brain extract in 1%SDS, 6M urea.
  • 常规说明

    This clone gives a much stronger signal in pig, cow and human samples than in rodents. For rodent samples use ab68428.

性能

应用

Our Abpromise guarantee covers the use of ab4648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA 1/5000.

Use this antibody as Capture antibody with Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) as Detection antibody at 0.5µg/ml.

ICC Use at an assay dependent concentration.
IHC-P 1/2000.
ICC/IF 1/10 - 1/50.
WB 1/100 - 1/500. Detects a band of approximately 55 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性Expressed in cells lacking fibronectin.
  • 疾病相关Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰Phosphorylated by PKN1.
  • 细胞定位Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody [2A5] 图像

  • ab4648 staining GFAP - Astrocyte Marker in pig brain tissue sections by IHC-Fr (formaldehyde-fixed Frozen sections). Tissue was fixed with formaldehyde (4%), permeabilized with Triton X-100 and blocked with 0.2% milk for 30 minutes at 24°C. Samples were incubated with primary antibody 1/2000 (TBS + 1% Triton X-100 + 0.2% milk) for 24 hours at 4°C. A Biotin-conjugated Sheep polyclonal to mouse IgG, dilution 1/400, was used as secondary antibody. Endogenous peroxidase was blocked with 0.3% H2O2 in methanol for 20 minutes.

    See Abreview

  • Rat neuron/glia cultures stained with nmouse monoclonal to GFAP ab 4648 (red).
  • Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
  • Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
  • Standard Curve for GFAP; dilution range 1 pg/ml to 1 µg/ml using Capture Antibody Mouse monoclonal [2A5] to GFAP - Astrocyte Marker (ab4648) at 1/5000 and Detector Antibody Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) at 0.5 µg/ml.

  • Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).

Anti-GFAP antibody [2A5] (ab4648)参考文献

This product has been referenced in:
  • Kang TW  et al. Growth arrest and forced differentiation of human primary glioblastoma multiforme by a novel small molecule. Sci Rep 4:5546 (2014). WB, ICC/IF . Read more (PubMed: 24989033) »
  • Meli L  et al. Three dimensional cellular microarray platform for human neural stem cell differentiation and toxicology. Stem Cell Res 13:36-47 (2014). WB ; Human . Read more (PubMed: 24816401) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (spinal cord)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization Yes - 0.1% Tween 20 in PBS
Specification spinal cord
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Chia-Li Lin

Verified customer

提交于 Nov 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RTP°C
Sample Mouse Cell (Mouse primary astrocyte)
Specification Mouse primary astrocyte
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Dr. Craig Beall

Verified customer

提交于 Sep 15 2014

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Embryonic rat cerebral cortex)
Specification Embryonic rat cerebral cortex
Fixative Paraformaldehyde
Permeabilization Yes - Ice cold methanol
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 26 2012

Thank you for contacting Abcam.

Earlier we discussed your sELISA with ab4648 as the capture and ab48050 as the detector. As we discussed, since you would like to use your streptavidin-gold conjugated secondary, a detector that is conjugated to...

Read More

The protocol used was as follows:
Buffers and Reagents:
Carbonate Coating Buffer (100 mM)
Antigen or antibody should be diluted in coating buffer to immobilize them to the wells:
3.03 g Na2CO3,
6.0 g NaHCO3
Make up to 1000...

Read More

We do recommend usingthe Sodium Citrate buffer (pH 6.0), however HIER are effective as well.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Permeabilization Yes - Triton X-100
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 24°C
Username

Mr. Marian Hruska-Plochan

Verified customer

提交于 Feb 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"