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RabMAb

Anti-Gephyrin抗体[EPR12650] (ab181382)

概述

  • 产品名称
    Anti-Gephyrin抗体[EPR12650]
    参阅全部 Gephyrin 一抗
  • 描述
    兔单克隆抗体[EPR12650] to Gephyrin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Gephyrin aa 1-100. The exact sequence is proprietary.
    Database link: Q9NQX3

  • 阳性对照
    • Fetal brain tissue lysate, 293T and SH-SY5Y cell lysates; Human brain tissue; 293 cells.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab181382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 93 kDa (predicted molecular weight: 80 kDa).

靶标

  • 功能
    Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
  • 通路
    Cofactor biosynthesis; molybdopterin biosynthesis.
  • 疾病相关
    Defects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
    Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
  • 序列相似性
    In the N-terminal section; belongs to the moaB/mog family.
    In the C-terminal section; belongs to the moeA family.
  • 细胞定位
    Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Domain E antibody
    • Domain G antibody
    • GEPH antibody
    • GEPH_HUMAN antibody
    • GPH antibody
    • GPHN antibody
    • GPHRYN antibody
    • KIAA1385 antibody
    • Molybdopterin molybdenumtransferase antibody
    • MPT adenylyltransferase antibody
    • MPT Mo-transferase antibody
    see all

图片

  • All lanes : Anti-Gephyrin antibody [EPR12650] (ab181382) at 1 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : GPHN (Gephyrin) knockout HAP1 whole cell lysate
    Lane 3 : MCF7 whole cell lysate
    Lane 4 : U2OS whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 80 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab181382 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab181382 was shown to recognize Gephyrin in wild-type HAP1 cells as signal was lost at the expected MW in GPHN (Gephyrin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPHN (Gephyrin) knockout samples were subjected to SDS-PAGE. Ab181382 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunofluorescent staining of 293 cells using anti-Gephyrin RabMAb (red) DAPI nuclear staining (blue)

  • Immunohistochemical analysis of parafiin embedded human brain tissue using anti-Gephyrin RabMAb

  • All lanes : Anti-Gephyrin antibody [EPR12650] (ab181382) at 1/1000 dilution

    Lane 1 : Fetal brain tissue lysate
    Lane 2 : 293T cell lysate
    Lane 3 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 80 kDa

文献

ab181382 has not yet been referenced specifically in any publications.

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