Generic Caspase Activity Assay试剂盒(Fluorometric - Red) (ab112131)

概述

  • 产品名称Generic Caspase Activity Assay试剂盒(Fluorometric - Red)
    参阅全部 Caspase 试剂盒
  • 测试
    1 x 100 test
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam Activity Assay kits are a set of tools for monitoring cellular functions. The activation of caspase is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD).

    ab112131 is designed to monitor cell apoptosis through measuring generic caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) activation in live cells with our red fluorescent TF5-VAD-FMK probe. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). The cell permeable and nontoxic TF5-VAD-FMK irreversibly binds to activated casepase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further activation but will not stop apoptosis from proceeding.

    ab112131 can be conveniently used with the common fluorescence instruments equipped with the light sources and filters Ex/Em = ~647/660 nm. The red label allows for direct detection of activated caspases in apoptotic cells by a flow cytometer. It can be used for the quantification of most activated caspases activities in apoptotic cells, or for the screening of caspases inhibitors. The kit provides all the essential components.

    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用适用于: Flow Cyt, Functional Studiesmore details

性能

  • 相关性Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • 细胞定位Cytoplasmic

应用

Our Abpromise guarantee covers the use of ab112131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Generic Caspase Activity Assay Kit (Fluorometric - Red) 图像

  • Jurkat cells were untreated (Blue) or with 20 μM camptothecin (Pink) in a 37oC, 5% CO2 incubator for 4-5 hours, and then dye loaded with TF5-VAD-FMK for 1 hour.

实验方案

Generic Caspase Activity Assay Kit (Fluorometric - Red) (ab112131)参考文献

ab112131 has not yet been referenced specifically in any publications.

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