Generic Caspase Activity Assay试剂盒(Fluorometric - Green) (ab112130)


  • 产品名称
    Generic Caspase Activity Assay试剂盒(Fluorometric - Green)
    参阅全部 Caspase 试剂盒
  • 检测方法
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam's Activity Assay kits are a set of tools for monitoring cellular functions. The activation of caspase is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). ab112130 uses TF2-VAD-FMK as a fluorescent indicator for most caspase activities. The cell permeable and nontoxic TF2-VAD-FMK irreversibly binds to activated caspase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. Within 15 minutes incubation, it starts to react with active caspase enzymes.

    ab112130 provides all the essential components with an optimized assay protocol. It is designed to detect cell apoptosis by measuring generic activation of caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) in live cells.

    ab112130 is used for either the quantification of most activated caspase activities in apoptotic cells or screening of caspase inhibitors. TF2-VAD-FMK, the green label reagent, allows for direct detection of activated caspases in apoptotic cells by a flow cytometer at Ex/Em = 488/520 nm.

    Visit our FAQs page for tips and troubleshooting.

  • 说明

    ab112130 should be stored dessicated.

  • 经测试应用
    适用于: Flow Cyt, Functional Studiesmore details
  • 平台


  • 相关性
    Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • 细胞定位


Our Abpromise guarantee covers the use of ab112130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Generic Caspase Activity Assay Kit (Fluorometric - Green) 图像

  • The increase in TF2-VAD-FMK (Component A) fluorescence intensity with the addition of Camptothecin in Jurkat cells.
    Jurkat cells were untreated (Blue) or with 20 μM camptothecin (Pink) in a 37 °C, 5 % CO2 incubator for 4-5 hours, and then dye loaded with TF2-VAD-FMK for 1 hour.


Generic Caspase Activity Assay Kit (Fluorometric - Green) (ab112130)参考文献

This product has been referenced in:
  • Chen CY  et al. Tenuifolide B from Cinnamomum tenuifolium Stem Selectively Inhibits Proliferation of Oral Cancer Cells via Apoptosis, ROS Generation, Mitochondrial Depolarization, and DNA Damage. Toxins (Basel) 8:N/A (2016). Read more (PubMed: 27827950) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

You are correct that you can your cells for up to 24hours at 4C after they have been fixed. After the cells have been fixed. The kit is intended to be used on live cells.

Please let me know if there is anyt...

Read More

Thank you forcontacting Abcam.

Youcan store your samples at 4C for up to 24hours, but you cannot freeze them.

Please let me know if there is anything else I can help you with.