Anti-Geminin抗体(ab84423)

概述

  • 产品名称Anti-Geminin抗体
    参阅全部 Geminin 一抗
  • 描述
    兔多克隆抗体to Geminin
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide: KRKHRNDHLT STTSSPGVIV PESSENKNLG GVTQESFDLM IKENPSSQYW K, corresponding to amino acids 50-100 of Human Geminin (NP_056979.1)

  • 阳性对照
    • Human breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab84423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer recommended.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
    • 序列相似性Belongs to the geminin family.
    • 发展阶段Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA replication inhibitor antibody
      • Gem antibody
      • GEMI_HUMAN antibody
      • Geminin antibody
      • Geminin DNA replication inhibitor antibody
      • GMNN antibody
      • OTTHUMP00000039393 antibody
      • RP3 369A17.3 antibody
      see all

    Anti-Geminin antibody 图像

    • ab84423 staining Geminin in Human tonsil tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in EDTA pH8.0 for 20 minutes at 100°C. Samples were incubated with primary antibody (1/40) for 20 minutes at 25°C. A HPR-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma using 1/250 ab84423, followed by DAB staining.

    Anti-Geminin antibody (ab84423)参考文献

    ab84423 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

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    Verified customer

    提交于 Nov 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (tonsil)
    Specification tonsil
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH8.0, 20 min, 100C
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    提交于 May 10 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"