Anti-Geminin抗体[1A8] (ab104306)
Key features and details
- Mouse monoclonal [1A8] to Geminin
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Geminin抗体[1A8]
参阅全部 Geminin 一抗 -
描述
小鼠单克隆抗体[1A8] to Geminin -
宿主
Mouse -
经测试应用
适用于: IHC-P, ICC/IF, WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 110-209 of Human Geminin (AAH05185) with a proprietary tag.
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阳性对照
- Partial tagged recombinant Geminin; Geminin transfected 293T cell lysate; Human lymph node; Human placenta; HeLa cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 22 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
1A8 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104306于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. -
序列相似性
Belongs to the geminin family. -
发展阶段
Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. - Information by UniProt
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数据库链接
- Entrez Gene: 51053 Human
- Omim: 602842 Human
- SwissProt: O75496 Human
- Unigene: 234896 Human
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别名
- DNA replication inhibitor antibody
- Gem antibody
- GEMI_HUMAN antibody
see all
图片
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Anti-Geminin antibody [1A8] (ab104306) at 5 µg/ml + Immunogen at 0.2 µg
Predicted band size: 24 kDaThe tagged immunogen has a predicted MWt of 37 kDa.
This image was generated using the ascites version of the product.
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All lanes : Anti-Geminin antibody [1A8] (ab104306) at 5 µg/ml
Lane 1 : Geminin transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 24 kDaThis image was generated using the ascites version of the product.
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ab104306, at 3 µg/ml, staining Geminin in formalin-fixed paraffin-embedded Human lymph node by Immunohistochemistry.
This image was generated using the ascites version of the product.
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ab104306, at 1µg/ml, staining Geminin in formalin-fixed paraffin-embedded Human placenta by Immunohistochemistry.
This image was generated using the ascites version of the product.
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ab104306, at 10 µg/ml, staining Geminin in HeLa cells by Immunofluorescence.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab104306 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104306, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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Immunofluorescence analysis of Caki-2 (Clear cell renal carcinoma) cells, staining Geminin with ab104306.
Cells were fixed with formaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 1% BSA for 30 minutes at 25oC. Primary antibody was concentrated using ab102778 Antibody Concentration Kit and conjugated to Rhodamine using ab102915 EasyLink Rhodamine Conjugation Kit. Samples were incubated with conjugated primary antibody (20 μg/ml in diluent) for 2 hours at 25oC.This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab104306 被引用在 7 文献中.
- Alenezi WM et al. The Genetic and Molecular Analyses of RAD51C and RAD51D Identifies Rare Variants Implicated in Hereditary Ovarian Cancer from a Genetically Unique Population. Cancers (Basel) 14:N/A (2022). PubMed: 35565380
- Zhao X et al. High expression of GMNN predicts malignant progression and poor prognosis in ACC. Eur J Med Res 27:301 (2022). PubMed: 36539849
- Yamanaka R et al. Cellular thermogenesis compensates environmental temperature fluctuations for maintaining intracellular temperature. Biochem Biophys Res Commun 533:70-76 (2020). PubMed: 32928506
- Kondrashova O et al. Methylation of all BRCA1 copies predicts response to the PARP inhibitor rucaparib in ovarian carcinoma. Nat Commun 9:3970 (2018). PubMed: 30266954
- Dhuppar S & Mazumder A Measuring cell cycle-dependent DNA damage responses and p53 regulation on a cell-by-cell basis from image analysis. Cell Cycle 17:1358-1371 (2018). PubMed: 29963960
- Kondrashova O et al. Secondary Somatic Mutations RestoringRAD51CandRAD51DAssociated with Acquired Resistance to the PARP Inhibitor Rucaparib in High-Grade Ovarian Carcinoma. Cancer Discov 7:984-998 (2017). PubMed: 28588062
- Jimenez-Hernandez M et al. Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro. Analyst N/A:N/A (2013). PubMed: 23640135