概述

  • 产品名称
    Anti-Geminin抗体
    参阅全部 Geminin 一抗
  • 描述
    小鼠单克隆抗体to Geminin
  • 经测试应用
    适用于: ELISA, IHC-P, ICC/IF, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 110-209 of Human Geminin (AAH05185) with a proprietary tag.

  • 阳性对照
    • Partial tagged recombinant Geminin; Geminin transfected 293T cell lysate; Human lymph node; Human placenta; HeLa cells.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab195047 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab104306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged Geminin is approximately 0.03ng/ml when used as a capture antibody.
IHC-P Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
  • 序列相似性
    Belongs to the geminin family.
  • 发展阶段
    Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA replication inhibitor antibody
    • Gem antibody
    • GEMI_HUMAN antibody
    • Geminin antibody
    • Geminin DNA replication inhibitor antibody
    • GMNN antibody
    • OTTHUMP00000039393 antibody
    • RP3 369A17.3 antibody
    see all

图片

  • Anti-Geminin antibody (ab104306) at 5 µg/ml + Immunogen at 0.2 µg

    Predicted band size : 24 kDa
  • All lanes : Anti-Geminin antibody (ab104306) at 5 µg/ml

    Lane 1 : Geminin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 24 kDa
  • ab104306, at 3 µg/ml, staining Geminin in formalin-fixed paraffin-embedded Human lymph node by Immunohistochemistry.
  • ab104306, at 1µg/ml, staining Geminin in formalin-fixed paraffin-embedded Human placenta by Immunohistochemistry.
  • ab104306, at 10 µg/ml, staining Geminin in HeLa cells by Immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab104306 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104306, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunofluorescence analysis of Caki-2 (Clear cell renal carcinoma) cells, staining Geminin with ab104306.

    Cells were fixed with formaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Primary antibody was concentrated using ab102778 Antibody Concentration Kit and conjugated to Rhodamine using ab102915 EasyLink Rhodamine Conjugation Kit. Samples were incubated with conjugated primary antibody (20 µg/ml in diluent) for 2 hours at 25°C.

    See Abreview

文献

This product has been referenced in:
  • Jimenez-Hernandez M  et al. Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro. Analyst N/A:N/A (2013). Read more (PubMed: 23640135) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us.

The following protocol was used with ab104306 for ICC/IF:

Preparation of slides:

1. Grow cultured cells on sterile glass cover slips at 37°C overnight.

2. Wash sample with PBS twice.<...

Read More
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (Caki-2 (Clear cell renal carcinoma))
Specification
Caki-2 (Clear cell renal carcinoma)
Permeabilization
Yes - 0.25% X-Triton in PBS
Fixative
Formaldehyde
Username

Dr. Melody Jimenez

Verified customer

提交于 Feb 05 2013

Thank you for your reply.

As I mentioned in my previous email, we do not have sample sizes of our products. Instead we guarantee them, under our Abpromise to work as stated on the datasheet. If for whatever reason they fail to work, then we wi...

Read More

Thank you for contacting Abccam. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

Read More

I'm glad to hear it. If you do have any further questions please do not hesitate to contact me again.

Until then, I wish you all the best with your experiments.

I am sorry that the protocol details were not explained clearly enough. I will try to explain as best I can, if it does not make sense please let me know and I'll try to rephrase.

You would take your antibody (concentration of 0.36 mg/mL), yo...

Read More

Thank you for confirming that information. I have arranged for thenew secondary antibody (ab96895) to be sent out to you directly (to the address specified in your last email). The new order number is xxxx(FOCRxxxxx). This should reach you later on tod...

Read More

I'm sorry for the delay in getting back to you, we have been closed over the Easter holidays.

I have had a look at the secondary antibodies you have suggested as trying as an alternative to the one currently used (ab60314). All seem fine for ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册