概述

  • 产品名称Anti-Gemin 5抗体
    参阅全部 Gemin 5 一抗
  • 描述
    兔多克隆抗体to Gemin 5
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    A region within amino acids 1360-1410 of Human Gemin 5 (NP_056280.1)

  • 阳性对照
    • HeLa whole cell lysate. 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab99360 was affinity purified using an epitope specific to Gemin 5 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab99360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 169 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性Gemin 5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN, Gemin 2 (SIP1), Gemin 3 (DDX20), and Gemin 4.
  • 细胞定位Nuclear and cytoplasmic
  • 数据库链接
  • 别名
    • gem (nuclear organelle) associated protein 5 antibody
    • Gem-associated protein 5 antibody
    • GEMIN5 antibody

Anti-Gemin 5 antibody 图像

  • All lanes : Anti-Gemin 5 antibody (ab99360) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 169 kDa


    Exposure time : 10 seconds
  • ab99360 at 1 µg/ml detecting Gemin 5 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 1 second exposure.
    For immunoprecipitation, ab99360 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99360
    Lane 2; IP using control IgG.

Anti-Gemin 5 antibody (ab99360)参考文献

ab99360 has not yet been referenced specifically in any publications.

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