兔多克隆抗体to Gemin 4
Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 1010 and the C-terminus (residue 1058) of Human Gemin 4 (NP_056536.1).
Whole cell lysate from HeLa and 293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 120 kDa).
Use at 2-5 µg/mg of lysate.
Anti-Gemin 4 antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 4 antibody (ab70750)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 4 with ab70750 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Gemin 4 antibody (ab70750)
All lanes :
Anti-Gemin 4 antibody (ab70750) at 1/5000 dilution
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size :
Observed band size :
117 kDa (
why is the actual band size different from the predicted?
Additional bands at :
160 kDa,270 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
Immunoprecipitation - Gemin 4 antibody (ab70750)
Detection of Gemin 4 by Western Blot of Immunprecipitate.
ab70750 at 1µg/ml staining Gemin 4 in HeLa whole cell lysate immunoprecipitated using ab70750 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds.
Anti-Gemin 4 antibody (ab70750)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"