Anti-Gemin 4抗体(ab70750)

概述

  • 产品名称Anti-Gemin 4抗体
    参阅全部 Gemin 4 一抗
  • 描述
    兔多克隆抗体to Gemin 4
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1010 and the C-terminus (residue 1058) of Human Gemin 4 (NP_056536.1).

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab70750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 120 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性GEMIN 4 is part of the SMN complex and plays an essential role in spliceosomal snRNP assembly in the cytoplasm. It is required for pre mRNA splicing in the nucleus.
  • 细胞定位Cytoplasmic and nuclear.
  • 数据库链接
  • 别名
    • Component of gems 4 antibody
    • Gem associated protein 4 antibody
    • HC56 antibody
    • HHRF 1 antibody

Anti-Gemin 4 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 4 with ab70750 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Gemin 4 antibody (ab70750) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 120 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,270 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Gemin 4 by Western Blot of Immunprecipitate. ab70750 at 1µg/ml staining Gemin 4 in HeLa whole cell lysate immunoprecipitated using ab70750 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.

Anti-Gemin 4 antibody (ab70750)参考文献

ab70750 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Lymphocyte)
Gel Running Conditions Reduced Denaturing (4-12% bis-tris)
Loading amount 40 µg
Specification Lymphocyte
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"