兔多克隆抗体to Gemin 4
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Gemin 4.
(Peptide available as
ab31581 gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line),
Jurkat (Human T cell lymphoblast-like cell line),
A431 (Human epithelial carcinoma cell line),
HEK 293 (Human embryonic kidney cell line),
MCF-7 (Human breast adenocarcinoma cell line),
SHSY-5Y (Human neuroblastoma cell line).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
Use a concentration of 5 µg/ml.
Anti-Gemin 4 antibody 图像
Western blot - Anti-Gemin 4 antibody (ab31581)
All lanes :
Anti-Gemin 4 antibody (ab31581) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat whole cell lysate ( ab7899) Lane 3 : A431 whole cell lysate ( ab7909) Lane 4 : HEK293 whole cell lysate ( ab7902) Lysates/proteins at 10 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution Performed under reducing conditions. Predicted band size : 120 kDa Observed band size : 110 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 4 antibody (ab31581)
ICC/IF image of ab31581 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31581, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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