概述

  • 产品名称Anti-Gemin 3抗体
    参阅全部 Gemin 3 一抗
  • 描述
    小鼠多克隆抗体to Gemin 3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human Gemin 3 protein (AAH34953.1).

  • 阳性对照
    • Human placenta tissue lysate, Gemin 3 transfected 293T cell lysate and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the DEAD box helicase family. DDX20 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 细胞定位Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Component of gems 3 antibody
    • DDX 20 antibody
    • ddx20 antibody
    • DDX20_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 20 antibody
    • DEAD box protein 20 antibody
    • DEAD box protein DP 103 antibody
    • DEAD box protein DP103 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 antibody
    • DEAD/H BOX 20 antibody
    • DKFZP434H052 antibody
    • DP 103 antibody
    • DP103 antibody
    • Gemin-3 antibody
    • Gemin3 antibody
    • Probable ATP dependent RNA helicase DDX20 antibody
    • Probable ATP-dependent RNA helicase ddx20 antibody
    • SMN interacting protein antibody
    see all

Anti-Gemin 3 antibody 图像

  • Anti-Gemin 3 antibody (ab88863) at 1 µg/ml + Human placenta tissue lysate at 50 µg

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 125 kDa,250 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Gemin 3 antibody (ab88863) at 1 µg/ml

    Lane 1 : Gemin 3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 92 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
  • ab88863, at 10 µg/ml staining Gemin 3 in HeLa cells by Immunofluorescence.

Anti-Gemin 3 antibody (ab88863)参考文献

ab88863 has not yet been referenced specifically in any publications.

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