RabMAb

Anti-Gemin 1抗体[EPR4429] (ab108531)

概述

  • 产品名称Anti-Gemin 1抗体[EPR4429]
    参阅全部 Gemin 1 一抗
  • 描述
    兔单克隆抗体[EPR4429] to Gemin 1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Gemin 1 aa 50-150 (internal sequence).

  • 阳性对照
    • WB: HeLa, HepG2, K562, HEK293 and 293T cell lysates. ICC/IF: SH-SY5Y cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250.

For unpurified use at 1/100 - 1/250.

WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

For unpurified use at 1/1000 - 1/10000.

靶标

  • 功能The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
  • 组织特异性Expressed in a wide variety of tissues. Expressed at high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes. Also seen at high levels in spinal cord. Present in osteoclasts and mononuclear cells (at protein level).
  • 疾病相关Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 1 (SMA1) [MIM:253300]. Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. Autosomal recessive forms are classified according to the age of onset, the maximum muscular activity achieved, and survivorship. The severity of the disease is mainly determined by the copy number of SMN2, a copy gene which predominantly produces exon 7-skipped transcripts and only low amount of full-length transcripts that encode for a protein identical to SMN1. Only about 4% of SMA patients bear one SMN1 copy with an intragenic mutation. SMA1 is a severe form, with onset before 6 months of age. SMA1 patients never achieve the ability to sit.
    Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 2 (SMA2) [MIM:253550]. SMA2 is an autosomal recessive spinal muscular atrophy of intermediate severity, with onset between 6 and 18 months. Patients do not reach the motor milestone of standing, and survive into adulthood.
    Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 3 (SMA3) [MIM:253400]. SMA3 is an autosomal recessive spinal muscular atrophy with onset after 18 months. SMA3 patients develop ability to stand and walk and survive into adulthood.
    Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 4 (SMA4) [MIM:271150]. SMA4 is an autosomal recessive spinal muscular atrophy characterized by symmetric proximal muscle weakness with onset in adulthood and slow disease progression. SMA4 patients can stand and walk.
  • 序列相似性Belongs to the SMN family.
    Contains 1 Tudor domain.
  • 细胞定位Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCD541 antibody
    • Component of gems 1 antibody
    • Gemin 1 antibody
    • Gemin-1 antibody
    • OTTHUMP00000125198 antibody
    • OTTHUMP00000223567 antibody
    • OTTHUMP00000223568 antibody
    • OTTHUMP00000224066 antibody
    • OTTHUMP00000226924 antibody
    • SMA 1 antibody
    • SMA 2 antibody
    • SMA 3 antibody
    • SMA 4 antibody
    • SMA antibody
    • SMA@ antibody
    • SMA1 antibody
    • SMA2 antibody
    • SMA3 antibody
    • SMA4 antibody
    • SMN antibody
    • SMN_HUMAN antibody
    • SMN1 antibody
    • SMN2 antibody
    • SMNT antibody
    • Survival motor neuron protein antibody
    • Survival of motor neuron 1, telomeric antibody
    • T-BCD541 antibody
    see all

Anti-Gemin 1 antibody [EPR4429] 图像

  • Anti-Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (purified) + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (purified)

    Lane 1 : K562 cell lysate
    Lane 2 : HEK293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-Gemin 1 antibody [EPR4429] (ab108531) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size : 32 kDa
  • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Gemin 1 with purified ab108531 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

Anti-Gemin 1 antibody [EPR4429] (ab108531)参考文献

ab108531 has not yet been referenced specifically in any publications.

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