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A synthetic peptide corresponding to residues in the N terminal region of human Gelsolin.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab75832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/25000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
Bloking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lane 5-8: ab109014 detected both secretory and cytoplasmic isoforms. Lane 9-12: ab134183 detected both secretory and cytoplasmic isoforms.
ab75832 Immunoprecipitating Gelsolin in mouse embryo whole cell lysate. 2µg of capture antibody (ab75832) per 0.35mg of lysate was used. 10µg of plasma was incubated with primary antibody (Panel A: ab75832, Panel B: ab134183) at a dilution of 1/1000 and VeriBlot for IP secondary antibody (HRP) ab131366 was used as the secondary antibody at a dilution of 1/10000.
Lane 1: Human plasma
Lane 2: Human plasma
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.
Exposure time: Panel A - 8 seconds, Panel B - 3 minutes.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The additional band at 50 kDa is a rabbit IgG heavy chain.