概述

  • 产品名称Anti-Gelsolin抗体
    参阅全部 Gelsolin 一抗
  • 描述
    兔多克隆抗体to Gelsolin
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region of Human GSN

  • 阳性对照
    • MCF cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab76418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • 组织特异性Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • 疾病相关Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • 序列相似性Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • 翻译后修饰Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • 细胞定位Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

Anti-Gelsolin antibody 图像

  • ab76418 at 5µg/ml staining Gelsolin in tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-Gelsolin antibody (ab76418) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa


  • Predicted band size : 86 kDa
    Commercial M1 and M2 are cell culture supernatants of human monocyte derived macrophages.

Anti-Gelsolin antibody (ab76418)参考文献

This product has been referenced in:
  • Bewley MA  et al. Proteomic evaluation and validation of cathepsin D regulated proteins in macrophages exposed to Streptococcus pneumoniae. Mol Cell Proteomics 10:M111.008193 (2011). WB ; Human . Read more (PubMed: 21474794) »
  • Haverland N  et al. Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation. J Transl Med 8:137 (2010). WB ; Human . Read more (PubMed: 21171974) »

See all 2 Publications for this product

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