概述

  • 产品名称
    Anti-Gelsolin抗体
    参阅全部 Gelsolin 一抗
  • 描述
    山羊多克隆抗体to Gelsolin
  • 特异性
    This antibody is expected to recognize both reported isoforms (NP_000168.1 and NP_937895.1).
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Gelsolin aa 655-666 (internal sequence).
    Sequence:

    C-PRLKDKKMDAHP

  • 阳性对照
    • Human Spleen lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).

靶标

  • 功能
    Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • 组织特异性
    Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • 疾病相关
    Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • 序列相似性
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • 翻译后修饰
    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • 细胞定位
    Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

图片

  • Anti-Gelsolin antibody (ab53560) at 0.1 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer).

    Predicted band size : 86 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

实验方案

文献

ab53560 has not yet been referenced specifically in any publications.

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