概述

  • 产品名称Anti-GEFT抗体
    参阅全部 GEFT 一抗
  • 描述
    兔多克隆抗体to GEFT
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Spider Monkey
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 504-553 (RIRLGDQAQGSTHTPINGSLPSLLLSPKGEVARALLPLDKQALGDIPQA P) of Human GEFT (NP_891992).

  • 阳性对照
    • Fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab81684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.
  • 组织特异性Isoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver.
  • 序列相似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • 结构域The guanine nucleotide exchange activity is autoinhibited by the PH domain.
  • 细胞定位Cell membrane. Cytoplasm > myofibril > sarcomere. Highly colocalizes with actin regions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHGEF25 antibody
    • ARHGP_HUMAN antibody
    • GEFT antibody
    • Guanine nucleotide exchange factor GEFT antibody
    • p63RhoGEF antibody
    • RAC/CDC42 exchange factor antibody
    • Rac/Cdc42/Rho exchange factor GEFT antibody
    • Rho guanine nucleotide exchange factor 25 antibody
    • RhoA/RAC/CDC42 exchange factor antibody
    • RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT antibody
    see all

Anti-GEFT antibody 图像

  • Anti-GEFT antibody (ab81684) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted 1/50,000 - 1/100,000 as secondary antibody.

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Anti-GEFT antibody (ab81684)参考文献

ab81684 has not yet been referenced specifically in any publications.

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