Anti-GEF H1 (phospho S885)抗体(ab74156)
Key features and details
- Rabbit polyclonal to GEF H1 (phospho S885)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GEF H1 (phospho S885)抗体
参阅全部 GEF H1 一抗 -
描述
兔多克隆抗体to GEF H1 (phospho S885) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human GEF H1 (phospho S885).
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阳性对照
- Extracts from HeLa cells treated with TSA (400nM, 24hours) and Jurkat cells treated with forskolin (40nM, 30mins).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab74156 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74156于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 120 kDa.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 120 kDa. |
靶标
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功能
Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. -
序列相似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger. -
结构域
The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.
The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions. -
翻译后修饰
Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. -
细胞定位
Cytoplasm. Cell junction > tight junction. Golgi apparatus. Cytoplasm > cytoskeleton > spindle. Cell projection > ruffle membrane. Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 9181 Human
- Entrez Gene: 16800 Mouse
- Omim: 607560 Human
- SwissProt: Q92974 Human
- SwissProt: Q60875 Mouse
- Unigene: 516790 Human
- Unigene: 239329 Mouse
- Unigene: 482396 Mouse
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别名
- AA408978 antibody
- ARHG2 antibody
- ARHG2_HUMAN antibody
see all
图片
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All lanes : Anti-GEF H1 (phospho S885) antibody (ab74156) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with TSA (400nM, 24hours)
Lane 2 : Extracts from Jurkat cells treated with
forskolin (40nM, 30mins)
Lane 3 : Extracts from HeLa cells treated
with TSA (400nM, 24hours) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 120 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab74156 被引用在 3 文献中.
- Kashyap AS et al. GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses. Cell Rep 28:3367-3380.e8 (2019). PubMed: 31553907
- Dan Q et al. Claudin-2 suppresses GEF-H1, RHOA, and MRTF, thereby impacting proliferation and profibrotic phenotype of tubular cells. J Biol Chem 294:15446-15465 (2019). PubMed: 31481470
- Chiang HS et al. GEF-H1 controls microtubule-dependent sensing of nucleic acids for antiviral host defenses. Nat Immunol 15:63-71 (2014). PubMed: 24270516