Anti-GEF H1抗体[B4/7] (ab90783)

概述

  • 产品名称Anti-GEF H1抗体[B4/7]
    参阅全部 GEF H1 一抗
  • 描述
    小鼠单克隆抗体[B4/7] to GEF H1
  • 经测试应用适用于: IHC-P, WB, IP, IHC-Fr, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Dog, Human
  • 免疫原

    Protein fraction isolated from detergent extracts of MDCK cells.

性能

应用

Our Abpromise guarantee covers the use of ab90783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB 1/50. Predicted molecular weight: 112 kDa.
IP Use at an assay dependent concentration.
IHC-Fr 1/50.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA.
  • 序列相似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • 结构域The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.
    The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions.
  • 翻译后修饰Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules.
  • 细胞定位Cytoplasm. Cell junction > tight junction. Golgi apparatus. Cytoplasm > cytoskeleton > spindle. Cell projection > ruffle membrane. Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA408978 antibody
    • ARHG2 antibody
    • ARHG2_HUMAN antibody
    • ARHGEF 2 antibody
    • ARHGEF-2 antibody
    • ARHGEF2 antibody
    • GEF antibody
    • GEF H1 antibody
    • GEF-H1 antibody
    • GEFH1 antibody
    • Guanine nucleotide exchange factor H1 antibody
    • KIAA0651 antibody
    • Lbcl1 antibody
    • Lfc antibody
    • LFP40 antibody
    • MGC95068 antibody
    • Microtubule-regulated Rho-GEF antibody
    • mKIAA0651 antibody
    • P40 antibody
    • Proliferating cell nucleolar antigen p40 antibody
    • Protein GEF-H1 antibody
    • Rho guanine nucleotide exchange factor 2 antibody
    • rho/rac guanine nucleotide exchange factor (GEF) 2 antibody
    • rho/rac guanine nucleotide exchange factor 2 antibody
    • rho/rac guanine nucleotide exchange factor antibody
    see all

Anti-GEF H1 antibody [B4/7] 图像

  • IHC image of ab90783 staining in Hu breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90783, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Overlay histogram showing HeLa cells stained with ab90783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab90783, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-GEF H1 antibody [B4/7] (ab90783)参考文献

This product has been referenced in:
  • Tonami K  et al. Calpain-6, a microtubule-stabilizing protein, regulates Rac1 activity and cell motility through interaction with GEF-H1. J Cell Sci 124:1214-23 (2011). WB, ICC/IF ; Mouse . Read more (PubMed: 21406564) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: microwave
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Feb 15 2012

Thank you for your phone call.

I am sorry to hear that ab90783 did not work in rat in IHC with PFA fixed sections. Since this species and IHC technique had not been tested, I'd be happy to offer you a testing discount retrospectively as a on...

Read More

Thank you for your reply and your patience. I have contacted the laboratory producing the ab103558 in regards to the recognition of the phospho and the non-phospho form. The lab replied that there is a residue which can be reportedly phosphorylated wit...

Read More

Thank you for contacting us. I am sorry to hear that you are experiencing problems with ab90783. To my knowledge, the epitope of the ab90783 has not been determined. It is a monoclonal antibody generated against a cell fraction. I have not been aware t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cells)
Loading amount 30 µg
Specification 293 cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Melanie Blasius

Verified customer

提交于 Nov 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293 cells)
Total protein in input 1000 µg
Specification 293 cells
Immuno-precipitation step Other - protein G dynabeads
Username

Dr. Melanie Blasius

Verified customer

提交于 Nov 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"