• 产品名称Anti-GDNF抗体
    参阅全部 GDNF 一抗
  • 描述
    兔多克隆抗体to GDNF
  • 特异性GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Mouse GDNF.

    (Peptide available as ab74004.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HepG2 Whole Cell Mouse Liver Tissue Human Pancreas Tissue



Our Abpromise guarantee covers the use of ab64337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.


  • 功能Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • 组织特异性In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
  • 疾病相关Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
    Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • 序列相似性Belongs to the TGF-beta family. GDNF subfamily.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Astrocyte derived trophic factor antibody
    • Astrocyte derived trophic factor 1 antibody
    • Astrocyte-derived trophic factor antibody
    • ATF 1 antibody
    • ATF 2 antibody
    • Atf antibody
    • ATF1 antibody
    • ATF2 antibody
    • gdnf antibody
    • GDNF_HUMAN antibody
    • Glial cell derived neurotrophic factor antibody
    • Glial Cell Line Derived Neurotrophic Factor antibody
    • Glial cell line-derived neurotrophic factor antibody
    • Glial derived neurotrophic factor antibody
    • HFB1 GDNF antibody
    • hGDNF antibody
    • HSCR3 antibody
    see all

Anti-GDNF antibody 图像

  • All lanes : Anti-GDNF antibody (ab64337) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Human pancreas tissue lysate - total protein (ab29816)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-GDNF antibody (ab64337)参考文献

ab64337 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your reply and for the additional information.

Please keep me updated about any new results after heating the samples for 10 min and diluting the antibody to 1:1000.

I look forward to hearing from you, and le...

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Thank you for your reply and for sending the additional information. I apologize for the delay getting back to you, as our office was closed yesterday for Memorial Day.

Could you also tell me how longthe samples wereboiled in sample buffer, a...

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Thank you for your call today and for sending the image and information.

The band around 25 kDa is probably the expected monomer of GDNF, and the 50 kDa band is most likely the dimerized form of the protein. How long were the samples boiled i...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (embryonic kidney)
Specification embryonic kidney
Fixative PFA or MeOH or Acetone
Permeabilization No
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Odysse Michos

Verified customer

提交于 May 17 2010