概述

  • 产品名称Anti-GDI2抗体
    参阅全部 GDI2 一抗
  • 描述
    兔多克隆抗体to GDI2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Dog, Pig, Caenorhabditis elegans
  • 免疫原

    A region within synthetic peptide: EPEKEIRPAL ELLEPIEQKF VSISDLLVPK DLGTESQIFI SRTYDATTHF, corresponding to amino acids 361-410 of Human GDI2

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab49193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-GDI2 antibody 图像

  • All lanes : Anti-GDI2 antibody (ab49193) at 0.5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

Anti-GDI2 antibody (ab49193)参考文献

This product has been referenced in:
  • Hibbert B  et al. Glycogen synthase kinase-3ß inhibition augments diabetic endothelial progenitor cell abundance and functionality via cathepsin B: a novel therapeutic opportunity for arterial repair. Diabetes 63:1410-21 (2014). WB ; Human . Read more (PubMed: 24296714) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (astrocytroma)
Loading amount 25 µg
Specification astrocytroma
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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提交于 Apr 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"