Anti-GDF8 / Myostatin抗体(ab98337)

概述

  • 产品名称
    Anti-GDF8 / Myostatin抗体
    参阅全部 GDF8 / Myostatin 一抗
  • 描述
    兔多克隆抗体to GDF8 / Myostatin
  • 特异性
    This antibody is predicted to cross react with GDF11.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Goat, Horse, Chicken, Human
    预测可用于: Rat, Sheep, Turkey, Pig, Chimpanzee, Duck, Zebrafish, Baboon, Cynomolgus monkey, Goose, Macaque monkey, Quail, Gorilla, Catfish, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human GDF8/ Myostatin.

  • 阳性对照

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

靶标

Anti-GDF8 / Myostatin antibody 图像

  • All lanes : Anti-GDF8 / Myostatin antibody (ab98337) at 1/250 dilution

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 28 kDa (possible cleavage fragment).

    Exposure time : 16 minutes
  • All lanes : Anti-GDF8 / Myostatin antibody (ab98337) at 1/250 dilution

    Lane 1 : Recombinant human GDF8 / Myostatin protein (ab73251) at 0.1 µg
    Lane 2 : Recombinant human GDF8 / Myostatin protein (ab73251) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa


    Exposure time : 30 seconds

    ab73251 is a homodimer consisting of two 109aa monomers. The homodimer expected size is 25kDa, so the observed band is thought to be the monomer form.

实验方案

Anti-GDF8 / Myostatin antibody (ab98337)参考文献

This product has been referenced in:
  • Yu B  et al. Efficient TALEN-mediated myostatin gene editing in goats. BMC Dev Biol 16:26 (2016). WB ; Goat . Read more (PubMed: 27461387) »
  • Morrison PK  et al. Post-mortem stability of RNA in skeletal muscle and adipose tissue and the tissue-specific expression of myostatin, perilipin and associated factors in the horse. PLoS One 9:e100810 (2014). WB ; Horse . Read more (PubMed: 24956155) »

See all 4 Publications for this product

Product Wall

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Non-Denaturing (Native)
Sample
Human Cell lysate - whole cell (Gastrocnemius)
Specification
Gastrocnemius
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. Francesco Elia Marino

Verified customer

提交于 Mar 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10 % gel)
Sample
Chicken Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Horse Tissue lysate - whole (skeletal muscle)
Loading amount
45 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (4%stacking, 10% running)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Pippa Morrison

Verified customer

提交于 Nov 12 2012

That is no problem. If you have any further questions, please do not hesitate to contact us.


As you are interested in participating in the testing discount scheme, I have generated a discount code for you to use (See below). Once you ha...

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Thank you for contacting us.


I have performed a sequence comparison of the peptide immunogen used to raise this antibody to that of equine myostatin (SwissProt reference http://www.uniprot.org/uniprot/Q9GM97). The sequences match 100%, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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