Anti-GDF3抗体- Aminoterminal end (ab38547)

概述

  • 产品名称Anti-GDF3抗体- Aminoterminal end
    参阅全部 GDF3 一抗
  • 描述
    兔多克隆抗体to GDF3 - Aminoterminal end
  • 特异性This antibody is specific for GDF 3 (N term).
  • 经测试应用适用于: ICC/IF, ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 60 aa of human GDF 3.

  • 阳性对照
    • Breast carcinoma tissue and mouse kidney tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab38547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

靶标

Anti-GDF3 antibody - Aminoterminal end 图像

  • Anti-GDF3 antibody - Aminoterminal end (ab38547) at 1/100 dilution + Mouse kidney tissue lysate

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38547 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • ICC/IF image of ab38547 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38547, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GDF3 antibody - Aminoterminal end (ab38547)参考文献

ab38547 has not yet been referenced specifically in any publications.

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