概述

  • 产品名称Anti-GDF15抗体
    参阅全部 GDF15 一抗
  • 描述
    山羊多克隆抗体to GDF15
  • 经测试应用适用于: WB, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide: QKTDTGVSLQTYDD, corresponding to C terminal amino acids 286-299 of Human GDF15

  • 阳性对照
    • Human prostate lysate. Lysate prepared from transfected HEK293 cells transiently expressing GDF15.

性能

应用

Our Abpromise guarantee covers the use of ab39999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ICC/IF 1/250.
IHC-Fr 1/200. See Abreview.

靶标

  • 组织特异性Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
  • 序列相似性Belongs to the TGF-beta family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GDF 15 antibody
    • GDF-15 antibody
    • Gdf15 antibody
    • GDF15_HUMAN antibody
    • Growth differentiation factor 15 antibody
    • Growth/differentiation factor 15 antibody
    • Macrophage inhibitory cytokine 1 antibody
    • MIC 1 antibody
    • Mic-1 antibody
    • MIC1 antibody
    • NAG 1 antibody
    • NAG-1 antibody
    • NAG1 antibody
    • NRG 1 antibody
    • NRG-1 antibody
    • NRG1 antibody
    • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
    • NSAID antibody
    • NSAID regulated protein 1 antibody
    • NSAID-activated gene 1 protein antibody
    • NSAID-regulated gene 1 protein antibody
    • PDF antibody
    • PLAB antibody
    • Placental bone morphogenetic protein antibody
    • Placental bone morphogenic protein antibody
    • Placental TGF beta antibody
    • Placental TGF-beta antibody
    • Prostate differentiation factor antibody
    • PTGF beta antibody
    • PTGFB antibody
    see all

Anti-GDF15 antibody 图像

  • ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • Anti-GDF15 antibody (ab39999) at 0.3 µg/ml + Human Prostate lysate in RIPA buffer at 35 µg

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-GDF15 antibody (ab39999) at 0.3 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing GDF15

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-GDF15 antibody (ab39999)参考文献

This product has been referenced in:
  • Jones AC  et al. Prostate field cancerization: deregulated expression of macrophage inhibitory cytokine 1 (MIC-1) and platelet derived growth factor A (PDGF-A) in tumor adjacent tissue. PLoS One 10:e0119314 (2015). IHC-P ; Human . Read more (PubMed: 25767870) »
  • Hegde NV  et al. Interrelationships between tissue iron status and erythropoiesis during postweaning development following neonatal iron deficiency in rats. Am J Physiol Gastrointest Liver Physiol 300:G470-6 (2011). WB ; Rat . Read more (PubMed: 21193529) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (15% gel)
Sample Human Cell lysate - whole cell (HELA)
Specification HELA
Treatment 10uM Tunicamycin for 24hrs
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (Bone Marrow)
Specification Bone Marrow
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (aortic plaque)
Specification aortic plaque
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Miss. Silke Vorwald

Verified customer

提交于 Jun 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"