Anti-GDF15抗体[7C12.B3.F2] (ab106112)

概述

  • 产品名称Anti-GDF15抗体[7C12.B3.F2]
    参阅全部 GDF15 一抗
  • 描述
    小鼠单克隆抗体[7C12.B3.F2] to GDF15
  • 特异性ab106112 specifically reacts with an H variant sequence of GDF15 protein.
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey
  • 免疫原

    A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.

  • 阳性对照
    • Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag.

性能

应用

Our Abpromise guarantee covers the use of ab106112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/150000.
WB 1/1000. Predicted molecular weight: 34 kDa.
ICC/IF 1/1000.

靶标

  • 组织特异性Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
  • 序列相似性Belongs to the TGF-beta family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GDF 15 antibody
    • GDF-15 antibody
    • Gdf15 antibody
    • GDF15_HUMAN antibody
    • Growth differentiation factor 15 antibody
    • Growth/differentiation factor 15 antibody
    • Macrophage inhibitory cytokine 1 antibody
    • MIC 1 antibody
    • Mic-1 antibody
    • MIC1 antibody
    • NAG 1 antibody
    • NAG-1 antibody
    • NAG1 antibody
    • NRG 1 antibody
    • NRG-1 antibody
    • NRG1 antibody
    • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
    • NSAID antibody
    • NSAID regulated protein 1 antibody
    • NSAID-activated gene 1 protein antibody
    • NSAID-regulated gene 1 protein antibody
    • PDF antibody
    • PLAB antibody
    • Placental bone morphogenetic protein antibody
    • Placental bone morphogenic protein antibody
    • Placental TGF beta antibody
    • Placental TGF-beta antibody
    • Prostate differentiation factor antibody
    • PTGF beta antibody
    • PTGFB antibody
    see all

Anti-GDF15 antibody [7C12.B3.F2] 图像

  • ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.

  • All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilution

    Lane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag
    Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag
    Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant
    Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant

    Secondary
    Peroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilution

    Performed under reducing conditions.

    Predicted band size : 34 kDa

Anti-GDF15 antibody [7C12.B3.F2] (ab106112)参考文献

ab106112 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (Human blood film)
Specification Human blood film
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Formaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Sep 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"