Anti-GCSF Receptor抗体[S1390] (ab19479)

概述

  • 产品名称Anti-GCSF Receptor抗体[S1390]
    参阅全部 GCSF Receptor 一抗
  • 描述
    小鼠单克隆抗体[S1390] to GCSF Receptor
  • 特异性Reactive with G-CSFR and does not cross-react with MBP or BSA (bovine serum albumin).
  • 经测试应用适用于: IHC-Fr, Flow Cyt, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Purified recombinant MBP-GCSFR fusion protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab19479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 92 kDa.

靶标

  • 功能Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
  • 组织特异性One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
  • 疾病相关Hereditary neutrophilia
  • 序列相似性Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 114 antibody
    • CD114 antibody
    • CD114 antigen antibody
    • Colony stimulating factor 3 receptor (granulocyte) antibody
    • Colony stimulating factor 3 receptor antibody
    • CSF 3R antibody
    • CSF3R antibody
    • CSF3R_HUMAN antibody
    • Csfgr antibody
    • G CSF R antibody
    • G-CSF receptor antibody
    • G-CSF-R antibody
    • GCSFR antibody
    • Granulocyte colony stimulating factor receptor antibody
    • Granulocyte colony-stimulating factor receptor antibody
    • OTTHUMP00000009703 antibody
    • OTTHUMP00000009704 antibody
    • OTTHUMP00000009705 antibody
    see all

Anti-GCSF Receptor antibody [S1390] 图像

  • ab19479 staining the GCSF Receptor (red) in Mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue were fixed with paraformaldehyde and blocked with 5% BSA for 1 hoaur at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 12 hours at 4°C. A Cy3®-conjugated Donkey anti-mouse polyclonal (1/500) was used as secondary antibody. DAPI staining in blue.

    See Abreview

  • Overlay histogram showing U937 cells stained with ab19479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19479, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-GCSF Receptor antibody [S1390] (ab19479)参考文献

ab19479 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Nov 13 2015

Thank you for your patience in awaiting our reply. I have checked all of our monoclonal G-CSF antibodies and none of them have been epitope mapped, so we do not know where they bind. The polyclonal antibodies, of course, will bind many places. Please l...

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Thank you for your inquiry.

All of the information we have for each of our products is listed on the online datasheet for your convenience andthese are updated as soon as any new information is brought to our attention.


Regar...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Specification testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, EDTA (separately)
Permeabilization Yes - 0.1% Triton X-100
Blocking step 5% serum, 3% BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Jun 14 2012

Thank you for your email.

Sure, I have added this code to your review. Thanks again!

DISCOUNT CODE: *** Expiration date: April 12, 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab19479 in IHC-P and include this code in the “Additional Comments” secti...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X-100/PBS
Blocking step Blockace as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 24 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"