The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Plays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF (By similarity). Targets TJP1 to cell junctions. In cortical neurons, may play a role in glutaminergic signal transduction through interaction with the NMDA receptor subunit GRIN1.
组织特异性Detected in heart, liver, skeletal muscle, placenta, small intestine, lung, prostate and testis.
序列相似性Belongs to the MYZAP family.
细胞定位Cytoplasm > cytoskeleton. Cell membrane. Cytoplasm > myofibril > sarcomere > I band. Cytoplasm > myofibril > sarcomere > Z line. Cell junction. Detected predominantly at the intercalated disk in cardiomyocytes, and at low levels on sarcomeric Z disks. Colocalizes with F-actin. Colocalizes with cortical actin.