概述

  • 产品名称
  • 描述
    兔多克隆抗体to GCNT1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-428 of Human GCNT1 (NP_001481.2).

  • 阳性对照
    • Human Pancreas Lysate and transfected 293T lysate.

性能

应用

Our Abpromise guarantee covers the use of ab102665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF 1/50.

靶标

  • 功能
    Forms critical branches in O-glycans.
  • 组织特异性
    Highly expressed in activated T-lymphocytes and myeloid cells.
  • 通路
    Protein modification; protein glycosylation.
  • 序列相似性
    Belongs to the glycosyltransferase 14 family.
  • 细胞定位
    Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-galactosyl-O-glycosyl-glycoprotein beta-1 antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Beta-1 antibody
    • Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase antibody
    • C2GNT antibody
    • C2GNT L antibody
    • C2GNT1 antibody
    • C2GnTL antibody
    • Core 2 branching enzyme antibody
    • Core 2 GNT antibody
    • Core 2-branching enzyme antibody
    • Core2 GlcNAc transferase antibody
    • Core2-GlcNAc-transferase antibody
    • GCNT 1 antibody
    • GCNT1 antibody
    • GCNT1_HUMAN antibody
    • glucosaminyl (N-acetyl) transferase 1 core 2 antibody
    • NACGT2 antibody
    see all

图片

  • Anti-GCNT1 antibody (ab102665) at 1/500 dilution + Human Pancreas Tissue Lysate at 50 µg

    Predicted band size : 50 kDa
  • All lanes : Anti-GCNT1 antibody (ab102665) at 1/500 dilution

    Lane 1 : GCNT1 transfected 293T cell line
    Lane 2 : Non-transfected 293T cell line

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 50 kDa

文献

This product has been referenced in:
  • Nolz JC & Harty JT IL-15 regulates memory CD8+ T cell O-glycan synthesis and affects trafficking. J Clin Invest 124:1013-26 (2014). WB ; Mouse . Read more (PubMed: 24509081) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (Mouse tracheal tissue)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Mouse tracheal tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jan 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (KG1a)
Specification
KG1a
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% saponin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Apr 26 2012

DISCOUNT CODE: ***
Expiration date: August 25, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab102665 in ICC/IFand include this code in the “Add...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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