概述

  • 产品名称Anti-GCNF抗体
    参阅全部 GCNF 一抗
  • 描述
    兔多克隆抗体to GCNF
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GCNF.

    (Peptide available as ab38815.)

  • 阳性对照
    • This antibody gave a positive signal in Testis (Human) Tissue Lysate - adult normal tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab38816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human GCNF peptide (ab38815).
IHC-P Use at an assay dependent concentration. PubMed: 22926843

靶标

  • 功能Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.
  • 组织特异性Shows highest expression in the germ cells of the adult testis.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR6 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GCNF 1 antibody
    • GCNF antibody
    • GCNF1 antibody
    • Germ cell nuclear factor antibody
    • hGCNF antibody
    • hRTR antibody
    • NR 61 antibody
    • NR61 antibody
    • nr6a1 antibody
    • NR6A1_HUMAN antibody
    • Nuclear receptor subfamily 6 group A member 1 antibody
    • Orphan nuclear receptor NR6A1 antibody
    • Retinoic acid receptor related testis associated receptor antibody
    • Retinoid receptor related testis specific receptor antibody
    • Retinoid receptor-related testis-specific receptor antibody
    • RTR antibody
    see all

Anti-GCNF antibody 图像

  • All lanes : Anti-GCNF antibody (ab38816) at 1/250 dilution

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human testis tissue lysate - total protein (ab30257) with Human GCNF peptide (ab38815) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 230 kDa (possible cross reactivity).
  • All lanes : Anti-GCNF antibody (ab38816) at 1/80 dilution

    Lane 1 : Cell extract of HAmGCNF expressing COS1 cells.
    Lane 2 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 20 µg
    Lane 3 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 20 µg
    Lane 4 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 40 µg
    Lane 5 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 40 µg

    Secondary
    Goat anti-rabbit (Santa Cruz) at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • IHC-P image of GCNF staining with ab38816 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab38816 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

Anti-GCNF antibody (ab38816)参考文献

This product has been referenced in:
  • Chalmel F  et al. Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility. Hum Reprod 27:3233-48 (2012). IHC-P ; Human, Rat . Read more (PubMed: 22926843) »
  • Bianco C  et al. Regulation of human Cripto-1 expression by nuclear receptors and DNA promoter methylation in human embryonal and breast cancer cells. J Cell Physiol : (2012). WB ; Human . Read more (PubMed: 23129342) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E13.5 murine placenta)
Permeabilization Yes - 0.5% Tween 20 in PBS, 15 minutes, room temperature
Specification E13.5 murine placenta
Blocking step 10% goat serum in PBS as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jun 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (ES cells)
Loading amount 20 µg
Specification ES cells
Treatment 1 µM retinoic acid for 1.5 days
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult testis)
Specification adult testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

提交于 Feb 13 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

提交于 Feb 11 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Ovary)
Specification Ovary
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Jun 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"