重组Anti-GCN2 (phospho T899)抗体[EPR2320Y] (ab75836)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2320Y] to GCN2 (phospho T899)
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GCN2 (phospho T899)抗体[EPR2320Y]
参阅全部 GCN2 一抗 -
描述
兔单克隆抗体[EPR2320Y] to GCN2 (phospho T899) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysates treated with Calyculin. Dot Blot: GCN2 (phospho T899) phospho peptide.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2320Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75836于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Dot blot |
1/1000.
|
|
WB | (10) |
1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 187 kDa).
For unpurified use at 1/500. |
说明 |
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Dot blot
1/1000. |
WB
1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 187 kDa). For unpurified use at 1/500. |
靶标
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功能
Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain. -
结构域
Kinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1. -
翻译后修饰
Autophosphorylated on threonine residues. - Information by UniProt
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数据库链接
- Entrez Gene: 440275 Human
- Omim: 609280 Human
- SwissProt: Q9P2K8 Human
- Unigene: 656673 Human
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别名
- E2AK4_HUMAN antibody
- Eif2ak4 antibody
- Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
see all
图片
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All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) grown in serum free media for 24hours, whole cell lysate
Lane 2 : HeLa grown in serum free media for 24hours, then treated with 100nM Calyculin A for 30min, whole cell lysate
Lane 3 : HeLa grown in serum free media for 24hours, then treated with 100nM Calyculin A for 30min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 187 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Dot blot analysis of GCN2 (phospho T899) phospho peptide (Lane 1) and GCN2 non-phospho peptide (Lane 2) labelling GCN2 (phospho T899) phospho peptide with ab75836 at a dilution of 1:1000 dilution (0.277μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
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All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution (purified)
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with Calyculin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 187 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/500 dilution (unpurified)
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 187 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (69)
ab75836 被引用在 69 文献中.
- Shi J et al. The PERK/PKR-eIF2α Pathway Negatively Regulates Porcine Hemagglutinating Encephalomyelitis Virus Replication by Attenuating Global Protein Translation and Facilitating Stress Granule Formation. J Virol 96:e0169521 (2022). PubMed: 34643429
- Furnish M et al. MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent Stress Signaling. Mol Cancer Res 20:607-621 (2022). PubMed: 34992146
- Cordova RA et al. GCN2 eIF2 kinase promotes prostate cancer by maintaining amino acid homeostasis. Elife 11:N/A (2022). PubMed: 36107759
- Urano Y et al. Integrated stress response is involved in the 24(S)-hydroxycholesterol-induced unconventional cell death mechanism. Cell Death Discov 8:406 (2022). PubMed: 36195595
- Brüggenthies JB et al. A cell-based chemical-genetic screen for amino acid stress response inhibitors reveals torins reverse stress kinase GCN2 signaling. J Biol Chem 298:102629 (2022). PubMed: 36273589