RabMAb

Anti-GCN2 (phospho T667)抗体[EPR2319Y] (ab68427)

概述

  • 产品名称Anti-GCN2 (phospho T667)抗体[EPR2319Y]
    参阅全部 GCN2 一抗
  • 描述
    兔单克隆抗体[EPR2319Y] to GCN2 (phospho T667)
  • 特异性Detects GCN2 only when phosphorylyated at threonine 667.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to residues surrounding threonine 667 of human GCN2.

  • 阳性对照
    • HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab68427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
IP 1/30.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.
    • 组织特异性Widely expressed.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
      Contains 2 protein kinase domains.
      Contains 1 RWD domain.
    • 结构域Kinase domain 1 is a degenerate kinase domain.
      RWD domain is reported to interact with GCN1L1.
    • 翻译后修饰Autophosphorylated on threonine residues.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E2AK4_HUMAN antibody
      • Eif2ak4 antibody
      • Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 4 antibody
      • GCN2 antibody
      • GCN2 eIF2alpha kinase antibody
      • GCN2 like protein antibody
      • GCN2-like protein antibody
      • KIAA1338 antibody
      • MGCN2 antibody
      see all

    Anti-GCN2 (phospho T667) antibody [EPR2319Y] 图像

    • All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution

      Lane 1 : HeLa cell lysate - untreated
      Lane 2 : HeLa cell lysate - treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size : 220 kDa
      Observed band size : 220 kDa
      Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.

    Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)参考文献

    ab68427 has not yet been referenced specifically in any publications.

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