重组Anti-GCN2 (phospho T667)抗体[EPR2319Y] (ab68427)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2319Y] to GCN2 (phospho T667)
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GCN2 (phospho T667)抗体[EPR2319Y]
参阅全部 GCN2 一抗 -
描述
兔单克隆抗体[EPR2319Y] to GCN2 (phospho T667) -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cells IP: HeLa cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2319Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68427于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
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IP |
1/30.
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说明 |
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WB
1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa). |
IP
1/30. |
靶标
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功能
Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain. -
结构域
Kinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1. -
翻译后修饰
Autophosphorylated on threonine residues. - Information by UniProt
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数据库链接
- Entrez Gene: 440275 Human
- Omim: 609280 Human
- SwissProt: Q9P2K8 Human
- Unigene: 656673 Human
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别名
- E2AK4_HUMAN antibody
- Eif2ak4 antibody
- Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
see all
图片
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All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 220 kDa
Observed band size: 220 kDa
Additional bands at: 180 kDa. We are unsure as to the identity of these extra bands. -
GCN2 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab68427 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab68427 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab68427 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab68427 被引用在 1 文献中.
- Zhong H et al. African Swine Fever Virus MGF110-7L Induces Host Cell Translation Suppression and Stress Granule Formation by Activating the PERK/PKR-eIF2α Pathway. Microbiol Spectr 10:e0328222 (2022). PubMed: 36377947