GCN1 (general control of amino acid synthesis yeast homolog) like antibody
GCN1 (general control of amino acid synthesis 1 yeast) like 1 antibody
GCN1 general control of amino acid synthesis 1 like 1 antibody
GCN1 general control of amino acid synthesis 1 like 1 (yeast) antibody
GCN1 like protein 1 antibody
Anti-Gcn1l1 antibody 图像
Western blot - Gcn1l1 antibody (ab86139)
All lanes : Anti-Gcn1l1 antibody (ab86139) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 293 kDa Observed band size : 293 kDa
Exposure time : 30 seconds
Immunoprecipitation - Gcn1l1 antibody (ab86139)
Detection of Human Gcn1l1 by Immunoprecipitation, using ab86139 at 3µg/mg lysate. Image shows immunoprecipitated Gcn1l1 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with ab86139 at 1 µg/ml in lane 1 and control IgG in lane 2. Detection: Chemiluminescence with an exposure time of 30 seconds.
ICC/IF image of ab86139 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86139, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab86139 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86139, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Gcn1l1 antibody (ab86139)参考文献
has not yet been referenced specifically in any publications.