概述

  • 产品名称
  • 描述
    兔多克隆抗体to GCM2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus GCM2.

    (Peptide available as ab69686.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: F9 whole cell Brain (Mouse) tissue Brain (Mouse) tissue, 0 days old Skeletal Muscle (Mouse) Tissue Thymus (Rat) Tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

靶标

  • 相关性
    Gcm2, a mouse ortholog of the Drosophila Glial Cells Missing gene, is expressed in the parathyroid-specific domains in the 3rd pouches from E9.5. The null mutation of Gcm2 causes aparathyroidism in the fetal and adult mouse and has been proposed to be a master regulator for parathyroid development. During Drosophila embryogenesis Gcm2 plays a crucial role in promoting glial cell differentiation.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • chorion specific transcription factor GCMb antibody
    • Chorion-specific transcription factor GCMb antibody
    • GCM motif protein 2 antibody
    • GCMb antibody
    • Glial cells missing 2 antibody
    • glial cells missing homolog 2 (Drosophila) antibody
    • Glial cells missing homolog 2 antibody
    • glial cells missing homolog b antibody
    • glide gcm protein homolog antibody
    • glide/gcm protein homolog antibody
    • hGCMb antibody
    see all

图片

  • All lanes : Anti-GCM2 antibody (ab64723) at 1 µg/ml

    Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : Brain (Mouse) Tissue, 0 days old
    Lane 3 : Skeletal Muscle (Mouse) Tissue
    Lane 4 : Thymus (Rat) Tissue
    Lane 5 : Thyroid (Mouse) Tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 37 kDa, 43 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64723 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

实验方案

文献

This product has been referenced in:
  • Bain VE  et al. Tissue-specific roles for sonic hedgehog signaling in establishing thymus and parathyroid organ fate. Development 143:4027-4037 (2016). Read more (PubMed: 27633995) »
  • Reeh KA  et al. Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis. Development 141:2950-8 (2014). Read more (PubMed: 25053428) »

See all 2 Publications for this product

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