概述

  • 产品名称Anti-GCM1抗体
    参阅全部 GCM1 一抗
  • 描述
    小鼠单克隆抗体to GCM1
  • 经测试应用适用于: WB, ELISA, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum
  • 免疫原

    Recombinant fragment corresponding to amino acids 108-166 (QQRKRCPNCD GPLKLIPCRG HGGFPVTNFW RHDGRFIFFQ SKGEHDHPKP ETKLEAEA*) of Human GCM1 (NP_003634) linked to a proprietary tag.

  • 阳性对照
    • FHs 173 whole extract. HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 49 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

Anti-GCM1 antibody 图像

  • Anti-GCM1 antibody (ab88748) at 5 µg/ml + FHs 173 WE cell lysate at 50 µg

    Predicted band size : 49 kDa
    Observed band size : ~52 kDa (why is the actual band size different from the predicted?)
  • ab88748 at 10 µg/ml staining GCM1 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.
  • ab88748 at 10 µg/ml detecting GCM1 by sandwich ELISA. The detection limit for GCM1, is approximately 0.03 ng/ml when using ab88748 as a capture antibody.
  • Overlay histogram showing JEG3 cells stained with ab88748 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88748, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-GCM1 antibody (ab88748)参考文献

ab88748 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (Mouse trophoblast stem cell)
Specification Mouse trophoblast stem cell
Treatment various concentrations of X (a drug) for 1 h
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Hongfeng Ruan

Verified customer

提交于 Aug 19 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Placentome)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization No
Specification Placentome
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Carnoy's fixative
Username

Mr. Jeremy Gingrich

Verified customer

提交于 Oct 30 2015

Application Western blot
Sample Sheep Tissue lysate - whole (Placenta, caruncle)
Gel Running Conditions Reduced Denaturing (10% PAG)
Loading amount 10 µg
Specification Placenta, caruncle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Jeremy Gingrich

Verified customer

提交于 Oct 30 2015

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (placenta)
Permeabilization No
Specification placenta
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 13 2015

Abreviews
Application Western blot
Sample Human Tissue lysate - whole (placenta)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification placenta
Blocking step 4% block from PE as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"