RabMAb

Anti-GCLM抗体[EPR6668] (ab124827)

概述

  • 产品名称Anti-GCLM抗体[EPR6668]
    参阅全部 GCLM 一抗
  • 描述
    兔单克隆抗体[EPR6668] to GCLM
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GCLM aa 200-300.

  • 阳性对照
    • HeLa, A673 and K562 cell lysates; Human muscle and liver tissue. Rat kidney. NIH/3T3 (mouse embryo) whole cell lysate. K562 (human chronic myelogenous leukemia) whole cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

应用

Our Abpromise guarantee covers the use of ab124827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250 - 1/500.

靶标

  • 组织特异性In all tissues examined. Highest levels in skeletal muscle.
  • 通路Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • 序列相似性Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gamma ECS regulatory subunit antibody
    • Gamma-ECS regulatory subunit antibody
    • Gamma-glutamylcysteine synthetase regulatory subunit antibody
    • GCLM antibody
    • GCS light chain antibody
    • GLCLR antibody
    • Glutamate cysteine ligase regulatory subunit antibody
    • Glutamate--cysteine ligase modifier subunit antibody
    • Glutamate--cysteine ligase regulatory subunit antibody
    • GSC light chain antibody
    • GSH0_HUMAN antibody
    see all

Anti-GCLM antibody [EPR6668] 图像

  • ab124827 immunoprecipitating GCLM. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: K562 (human chronic myelogenous leukemia) whole cell lysate (10ug)
    Lane 2: K562 (human chronic myelogenous leukemia) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124827 in K562 (human chronic myelogenous leukemia) whole cell lysate

  • ab124827 staining GCLM in HeLa (human cervix adenocarcinoma) cells by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • ab124827 staining GCLM in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab124827 and secondary antibody ab150077 respectively. DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • Anti-GCLM antibody [EPR6668] (ab124827) at 1/10000 dilution + NIH/3T3 (mouse embryo) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG)

    Predicted band size : 31 kDa

    Diluting and blocking buffer: 5% NFDM /TBST 

  • ab124827 staining GCLM in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • All lanes : Anti-GCLM antibody [EPR6668] (ab124827) at 1/10000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : Human skeletal muscle
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 31 kDa

    Diluting and blocking buffer: 5% NFDM/TBST

  • All lanes : Anti-GCLM antibody [EPR6668] (ab124827) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A673 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size : 31 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab124827, at 1/100 dilution, staining GCLM in paraffin-embedded Human muscle tissue by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-GCLM antibody [EPR6668] (ab124827)参考文献

ab124827 has not yet been referenced specifically in any publications.

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ab124827 has been tested in mouse and immunocytochemistry (ICC) so it should be suitable for your experiment needs.

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