概述

  • 产品名称Anti-GCLM抗体
    参阅全部 GCLM 一抗
  • 描述
    兔多克隆抗体to GCLM
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region within internal amino acids 180-229 QVKPNSNQVN LASCCVMPPD LTAFAKQFDI QLLTHNDPKE LLSEASFQEA of Human GCLM.

  • 阳性对照
    • MCF7 Cell Lysate

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应用

Our Abpromise guarantee covers the use of ab81445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with Human GCLM peptide (ab111661). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-GCLM antibody 图像



  • Predicted band size : 31 kDa
    Western blot analysis of 40µg Mouse heart lysate (lanes 1 and 2) labeling GCLM with ab81445 at 1:1000. A HRP-conjugated anti-rabbit (1:10000) was used as the secondary antibody.
  • Anti-GCLM antibody (ab81445) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Gel Concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GCLM with ab81445 at 2.5 µg/ml.

Anti-GCLM antibody (ab81445)参考文献

ab81445 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart (young & old mouse))
Loading amount 30 µg
Specification Heart (young & old mouse)
Gel Running Conditions Non-reduced Denaturing (10-12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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Mr. Mtuhtu S.Gounder

Verified customer

提交于 Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"