概述

  • 产品名称Anti-GCLC抗体
    参阅全部 GCLC 一抗
  • 描述
    兔多克隆抗体to GCLC
  • 特异性ab80841 recognizes the heavy subunit of GCLC.
  • 经测试应用适用于: WB, IP, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Monkey
    预测可用于: Xenopus laevis, Zebrafish, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide corresponding to Rat GCLC aa 295-313 (internal sequence).
    Sequence:

    CRWGVISASVDDRTREERG

  • 阳性对照
    • Jurkat, MAD109 and PC12 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab80841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
IP Use at 10 µg/mg of lysate. (Denatured, use Protein A).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

Anti-GCLC antibody 图像

  • Anti-GCLC antibody (ab80841) at 2.5 µg/ml

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • ICC/IF image of ab80841 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80841, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of GCLC staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80841, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GCLC antibody (ab80841)参考文献

This product has been referenced in:
  • Wang X  et al. Activation of the Nuclear Factor E2-Related Factor 2/Antioxidant Response Element Pathway is Neuroprotective Following Spinal Cord Injury. J Neurotrauma : (2011). WB ; Rat . Read more (PubMed: 21806470) »

See 1 Publication for this product

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