概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to GCLC
  • 经测试应用
    适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 528-638 of Human GCLC.

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab207777 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab55435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 73 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 通路
    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • 疾病相关
    Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
  • 序列相似性
    Belongs to the glutamate--cysteine ligase type 3 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 6.3.2.2 antibody
    • Gamma ECS antibody
    • Gamma glutamylcysteine synthetase antibody
    • Gamma-ECS antibody
    • Gamma-glutamylcysteine synthetase antibody
    • GCL antibody
    • Gclc antibody
    • GCS antibody
    • GCS heavy chain antibody
    • GLCL antibody
    • GLCLC antibody
    • Glutamate cysteine ligase catalytic subunit antibody
    • Glutamate--cysteine ligase catalytic subunit antibody
    • GSH1_HUMAN antibody
    see all

图片



  • Predicted band size : 73 kDa
  • ab55435 at 10 ug/ml staining GCLC in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing Jurkat cells stained with ab55435 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55435, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Liu X  et al. Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2. PeerJ 5:e3642 (2017). Read more (PubMed: 28785522) »
  • Gu F  et al. Impaired synthesis and antioxidant defense of glutathione in the cerebellum of autistic subjects: alterations in the activities and protein expression of glutathione-related enzymes. Free Radic Biol Med 65:488-96 (2013). Read more (PubMed: 23892356) »

See all 5 Publications for this product

客户评价及客户问答

Thank you for your inquiry.
The optimal dilution for antibodies are dependent on many factors and that is the reason why we provide a range of suggest dilutions.
The factors include: amount of total protein per lane expression level of the sp...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册