概述

  • 产品名称
    Anti-GCLC抗体
    参阅全部 GCLC 一抗
  • 描述
    兔多克隆抗体to GCLC
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GCLC.

    参阅Abcam的专有抗源政策

    (The immunizing peptide is available as ab41462)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Tissue Lysates: Liver, Kidney and Lung (Adenocarcinoma). This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab41463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

靶标

图片

  • Western blot - Anti-GCLC antibody (ab41463)
    Western blot - Anti-GCLC antibody (ab41463)
    All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Human lung tissue lysate (adenocarcinoma) - insoluble fraction (female, 80 years) (ab42923)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 73 kDa
    Additional bands at: 58 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Shanmugam G  et al. Constitutive activation of Nrf2 induces a stable reductive state in the mouse myocardium. Redox Biol 12:937-945 (2017). WB ; Mouse . Read more (PubMed: 28482326) »
  • Kumar RR  et al. Abrogation of Nrf2 impairs antioxidant signaling and promotes atrial hypertrophy in response to high-intensity exercise stress. J Transl Med 14:86 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27048381) »

See all 14 Publications for this product

发表研究结果有使用 ab41463？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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1 Review or Q&A

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Votes: Highest First

Inquiry: Hello there, we have used an antibody from you back in 2006, it's called gamma-glutamylcysteine synthetase with a catalog number Ab17926. however, when I run a search on Abcam website, it's not there, instead search came out with Ab41463. I wo...

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Thank you for contacting us.
Unfortunately, the antibody was discontinued by the supplying lab.
The immunogens are derived from very different positions (ab17926 from AA295-313 of rat, ab41463 within residues 50 - 150 of Human GCLC). However,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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