概述

  • 产品名称
  • 描述
    兔多克隆抗体to GCLC
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GCLC.

    (The immunizing peptide is available as ab41462)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Tissue Lysates: Liver, Kidney and Lung (Adenocarcinoma). This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab41463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

靶标

图片

  • All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Human lung tissue lysate (adenocarcinoma) - insoluble fraction (female, 80 years) (ab42923)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 58 kDa,60 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Shanmugam G  et al. Constitutive activation of Nrf2 induces a stable reductive state in the mouse myocardium. Redox Biol 12:937-945 (2017). WB ; Mouse . Read more (PubMed: 28482326) »
  • Kumar RR  et al. Abrogation of Nrf2 impairs antioxidant signaling and promotes atrial hypertrophy in response to high-intensity exercise stress. J Transl Med 14:86 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27048381) »

See all 14 Publications for this product

客户评价及客户问答

Thank you for contacting us.
Unfortunately, the antibody was discontinued by the supplying lab.
The immunogens are derived from very different positions (ab17926 from AA295-313 of rat, ab41463 within residues 50 - 150 of Human GCLC). However,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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