存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Concentration information loading...
纯度 Protein G purified
纯化说明 ab71627 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
1/50 - 1/100.
功能 Inhibits glucokinase by forming an inactive complex with this enzyme.
组织特异性 Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
序列相似性 Belongs to the GCKR family. Contains 2 SIS domains.
Information by UniProt
Anti-GCKR antibody - N-terminal 图像
Western blot - GCKR antibody - N-terminal (ab71627)
Anti-GCKR antibody - N-terminal (ab71627) at 1/100 dilution + A375 cell lysate at 12.5 µg Predicted band size : 69 kDa Observed band size : 69,70 kDa ( why is the actual band size different from the predicted?) Additional bands at : 33 kDa,40 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCKR antibody - N-terminal (ab71627)
ab71627 at 1/50 dilution, staining GCKR in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
Anti-GCKR antibody - N-terminal (ab71627)参考文献
has not yet been referenced specifically in any publications.
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